The study of thiosulfate oxidation multi-enzyme system in the green sulfur bacterium Chlorobaculum tepidum

绿色硫杆菌温热绿杆菌硫代硫酸盐氧化多酶系统的研究

• 批准号: 23570064
• 负责人: INOUE Kazuhito
• 金额: $ 3.41万
• 依托单位: Kanagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570064/
• 关键词: 光合成; 硫黄酸化; 電子供与体; 緑色硫黄細菌; 電子伝達系; 光化学反応中心; 電子供与系; 光合成細菌; システイン; 光合成電子供与系; チオ硫酸; 硫化水素

SoxYZ is the factors indispensable for thiosulfate-dependent reduction of cyt c-554 from the green sulfur bacterium Chlorobaculum tepidum. Heterodimeric SoxYZ that binds oxidized product of thiosulfate on the cysteinyl-SH residue of SoxY (SoxY-SH) as the intermediate (SoxY-S)-SSO3- to yield (SoxY-S)-SH. Some preparations of SoxYZ showed low activity, and pretreatment of SoxYZ with sulfide increased the activity. On the other hand, pretreatments of SoxzYZ with DTT, thiosulfate and sulfite had no significant effects on the activity. We measured the mass of SoxY by MALDI-mass spectrometry. Compared with the sulfide-treated SoxYZ (m/z12852), the peaks of SoxY of as prepared SoxYZ with low activity in the TOMES assay had a mass number larger by about 30-80 than that of the former. The main mass peaks of both thiosulfate-treated and the sulfite-treated SoxYs also were larger by about 30-100. These results suggest that the inactive SoxY possibly binds –SO3 group on the cysteinyl S.

SoxYZ是硫代硫酸盐依赖还原绿色硫磺细菌绿细菌Cyt c-554不可缺少的因子。将硫代硫酸盐的氧化产物键合在半胱氨基-SH残基上的异二聚SOXYZ(SOXY-SH)作为中间体(SOXY-S)-SSO3-，生成(SOXY-S)-SH。部分SoxYZ制剂表现出较低的活性，而用硫化物预处理SoxYZ可提高其活性。另一方面，用DTT、硫代硫酸盐和亚硫酸盐对SoxzYZ进行预处理对酶活性没有显著影响。我们用MALDI-MS测定了SOX的质量。与硫化处理的SoxYZ(m/z12852)相比，在TOMS分析中活性较低的制备的SoxYZ的SOX峰的质量数比硫化处理的SoxYZ大约30-80个。硫代硫酸盐处理的SOXY和亚硫酸盐处理的SoxY的主质谱峰也都大了约30-100。这些结果表明，非活性的SOX可能结合了半胱氨基S上的-SO3基团。

项目成果

Interaction of cytc and monomeric flavoprotein SoxF, which enhances thiosulfate oxidation activity of the core TOMES in the green sulfur bacterium Chlorobaculum tepidum

细胞色素c和单体黄素蛋白SoxF的相互作用，增强绿硫细菌温热绿杆菌核心TOMES的硫代硫酸盐氧化活性

• 发表时间: 2012
• 作者: 小池祥子;本間祐紀;志賀倫子;瀬尾悌介;櫻井英博;井上和仁
• 通讯作者: 井上和仁

Inorganic sulfur oxidizing system of the green sulfur bacterium Chlorobaculum tepidum

绿色硫细菌温热绿杆菌的无机硫氧化系统

• 发表时间: 2012
• 作者: Sakurai H.;Ogawa T.;Shiga M.;Koike S.;Seo D.;Inoue K.
• 通讯作者: Inoue K.

Effects of pretreatment of SoxYZ preparations with various reductants on subsequent thiosulfate oxidation activity of core TOMES in the green sulfur bacterium Chlorobaculum tepidum

不同还原剂预处理SoxYZ制剂对绿硫细菌温热绿杆菌核心TOMES后续硫代硫酸盐氧化活性的影响

• 发表时间: 2013
• 作者: Koike S.;Homma Y.;Seo D.;Sakurai H.;Inoue K.
• 通讯作者: Inoue K.

Interaction of cyt c and monomeric flavoprotein SoxF, which enhances thiosulfate activity of the core TOMES in the green sulfur bacterium Chlorobaculum tepidum.

细胞色素 c 和单体黄素蛋白 SoxF 的相互作用，增强绿硫细菌温热绿杆菌中核心 TOMES 的硫代硫酸盐活性。

• 发表时间: 2012
• 作者: S. Koike;Y. Honma;M. Shiga;D. Seo;H. Sakurai and K. Inoue
• 通讯作者: H. Sakurai and K. Inoue

Effects of pretreatment of SoxYZ preparations with various reductants on subsequent thiosulfate oxidation activity of core TOMES in the green sulfur bacterium Chlorobaculum tepidum.

用各种还原剂预处理 SoxYZ 制剂对绿硫细菌温热绿杆菌后续核心 TOMES 硫代硫酸盐氧化活性的影响。

• 发表时间: 2013
• 作者: S. Koike;Y. Honma;D. Seo;H. Sakurai and K. Inoue
• 通讯作者: H. Sakurai and K. Inoue