Longitudinal Analysis of Low pH Streptococci and Plaque Microbiome in Early Childhood Caries
儿童早期龋齿中低 pH 链球菌和菌斑微生物组的纵向分析
基本信息
- 批准号:10388260
- 负责人:
- 金额:$ 53.83万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-06-01 至 2023-08-31
- 项目状态:已结题
- 来源:
- 关键词:Acid-Base EquilibriumAgeAreaBacteriaBioinformaticsCarbohydratesChildClinicalDataData AnalysesDental EnamelDental HygieneDental PlaqueDental cariesDevelopmentEcologyElementsEpidemiologyEquilibriumEtiologyExperimental DesignsGenesGenomicsGroupingHealthIntegration Host FactorsIowaLeadLeftLesionLiteratureLongitudinal StudiesMinorityNutritionalOdontogenesisOral cavityOrganismOutcomePathologyPatternPediatric DentistryPhenotypePopulationPreventionPropertyProspective StudiesResearchResearch PriorityRiskRisk AssessmentRoleSamplingScoring MethodSiteStreptococcusStreptococcus Viridans GroupStreptococcus anginosusStreptococcus gordoniiStreptococcus mitisStreptococcus mutansStreptococcus oralisSubgroupTaxonomyTimeUncertaintyUnited States National Institutes of HealthUniversitiesagedalkalinitybasedietaryearly childhoodexhausthealth disparityhigh riskhigh risk populationimplementation scienceimprovedinnovationlongitudinal analysismembermicrobialmicrobiomemicrobiome compositionnovelnovel strategiesoral microbiomeoral plaqueoral streptococcipediatric departmentprospectivesound
项目摘要
ABSTRACT
The microbial etiology of dental caries remains a subject of debate with considerable differences of
opinion regarding the relative contributions of specific species or classes of bacteria. The `low pH streptococci'
is a designation given to atypical, highly acidogenic strains of oral streptococci originally thought to belong to the
primary plaque colonizing species S. oralis, S. mitis, S. gordonii, and S. anginosus. This sub-group of
streptococci is proposed to have considerable cariogenic potential, perhaps preceding, augmenting or even
overshadowing the contribution of the mutans streptococci (MS). However, assessing the role of the low pH
strep is not straightforward. Accurate assignment of species among the viridans streptococci is a challenge, and
the low pH phenotype cannot be ascertained by the gene-based strategies routinely employed in non-culture
based studies of the oral microbiome. In this application we propose a unique prospective study that combines
state-of-the-art bioinformatics with phenotypic analyses of low pH phenotypes to track changes in the plaque
microbiome, with a specific focus on the MS and non-MS low pH streptococci, as caries develop in children at
high risk for early childhood caries. Specifically, our objectives are to determine if there is ascendancy of non-
MS low pH streptococci within the plaque oral microbiome with potential shifts in composition of other organisms
during the transition from a sound enamel site to a carious enamel site. In addition, we propose a novel and
exciting approach of categorizing low pH streptococci based on their potential to produce alkaline products. We
believe that gaining a more comprehensive assessment of the low pH streptococcal species or taxa, and our
novel grouping of these organisms based on alkaline-producing phenotypes, as part of our determination of the
plaque microbiome composition has the potential to make significant contributions to implementation science.
To meet our objectives we propose the following aims: 1) Conduct an 18-month longitudinal study on high-caries
risk children aged 1-3 to determine changes in the overall plaque microbiome preceding caries development,
with a focus on the non-MS low pH streptococci; and 2) Use genomic sequencing to assign species identity or
taxonomical relatedness to the non-MS low pH streptococci recovered in Aim 1. By coalescing and analyzing
the data from the microbiome and low pH streptococci as caries develop we will define patterns or drivers of
caries or health that may provide a basis for advances in implementation science.
摘要
龋齿的微生物病因学仍然是一个争论的主题,
关于特定细菌物种或类别的相对贡献的意见。“低pH链球菌”
是一种给予非典型的、高度产酸的口腔链球菌菌株的名称,最初被认为属于
主要噬菌斑定殖种S. oralis,S. mitis、细叶山蚂蝗S. gordonii和S.心绞痛这一小组
链球菌被认为具有相当大的致龋潜力,可能在致龋之前、增强致龋甚至
掩盖了变形链球菌(MS)的贡献。然而,评估低pH值的作用
链球菌并不简单。草绿色链球菌之间的物种的准确分配是一个挑战,
低pH表型不能通过非培养中常规采用的基于基因的策略来确定
基于口腔微生物组的研究。在本申请中,我们提出了一项独特的前瞻性研究,
最先进的生物信息学,对低pH表型进行表型分析,以跟踪菌斑的变化
微生物组,特别关注MS和非MS低pH链球菌,因为儿童龋齿的发展
儿童早期龋齿风险高。具体来说,我们的目标是确定是否存在非-
菌斑口腔微生物组内的MS低pH链球菌,其他微生物的组成可能发生变化
在从健康的釉质部位到龋坏的釉质部位的过渡期间。此外,我们还提出了一个新的和
一种令人兴奋的方法,根据它们产生碱性产物的潜力对低pH值链球菌进行分类。我们
我相信,获得一个更全面的评估低pH值链球菌物种或类群,我们的
基于产碱表型对这些生物进行新的分组,作为我们确定
牙菌斑微生物组组成具有为实施科学做出重大贡献的潜力。
为了达到我们的目标,我们提出了以下目标:1)对高龋进行为期18个月的纵向研究
1-3岁的风险儿童,以确定龋齿发展之前整体菌斑微生物组的变化,
重点关注非MS低pH链球菌;和2)使用基因组测序来指定物种身份或
与目标1中回收的非MS低pH链球菌的分类学相关性。通过整合和分析
微生物群系和低pH链球菌的数据作为龋齿的发展,我们将定义模式或驱动因素,
龋齿或健康,可以提供实施科学进步的基础。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A Social Media Intervention for Promoting Oral Health Behaviors in Adolescents: A Non-Randomized Pilot Clinical Trial.
- DOI:10.3390/oral3020018
- 发表时间:2023-06
- 期刊:
- 影响因子:0
- 作者:Calderon SJ;Comnick CL;Villhauer A;Marshall T;Dahl JU;Banas JA;Drake DR
- 通讯作者:Drake DR
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Jeffrey A Banas其他文献
Identification and sequence analysis of a methylase gene in Porphyromonas gingivalis.
牙龈卟啉单胞菌甲基化酶基因的鉴定和序列分析。
- DOI:
- 发表时间:
1991 - 期刊:
- 影响因子:14.9
- 作者:
Jeffrey A Banas;Joseph J. Ferretti;Ann Progulske - 通讯作者:
Ann Progulske
Jeffrey A Banas的其他文献
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{{ truncateString('Jeffrey A Banas', 18)}}的其他基金
Probiotics for Prevention of Early Childhood Caries
益生菌预防儿童早期龋齿
- 批准号:
10892707 - 财政年份:2023
- 资助金额:
$ 53.83万 - 项目类别:
Site-Specific Non-Mutans Streptococci Etiologies of Dental Caries
龋齿的位点特异性非变形链球菌病因学
- 批准号:
8278764 - 财政年份:2012
- 资助金额:
$ 53.83万 - 项目类别:
Site-Specific Non-Mutans Streptococci Etiologies of Dental Caries
龋齿的位点特异性非变形链球菌病因学
- 批准号:
8450696 - 财政年份:2012
- 资助金额:
$ 53.83万 - 项目类别:
Streptococcus mutans strain susceptibility differences to host defense peptides
变形链球菌菌株对宿主防御肽的敏感性差异
- 批准号:
7890861 - 财政年份:2010
- 资助金额:
$ 53.83万 - 项目类别:
Streptococcus mutans strain susceptibility differences to host defense peptides
变形链球菌菌株对宿主防御肽的敏感性差异
- 批准号:
8084205 - 财政年份:2010
- 资助金额:
$ 53.83万 - 项目类别:
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