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Action of sex pheromone in Amphibia

两栖类性信息素的作用

基本信息

批准号：
14540634
负责人：
TOYODA Fumiyo
金额：
$ 2.3万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

1.A female attracting pheromone of the newt, "sodefrin", was considered to act through the olfactory organ of female since attraction to this substance was abolished by bilateral nostril plugging or nerve transection between the nasal cavity and the olfactory bulb. Electrophysiological studies revealed that sodefrin evoked a marked electro-olfactogram (EOG) response when applied to the central region of the vomeronasal epithelium of the female. It was ascertained that the axons of vomeronasal receptor cells in vomeronasal epithelium terminate in the accessory olfactory bulb. We observed the various electrophysiological responses at the region near the accessory olfactory bulb by sodefrin application to the female vomeronasal region.2.Effects of arginine vasotocin (AVT) on reproductive events in the male. AVT enhanced the expression of androgen-induced courtship behavior. AVT was likely to act centrally since the behavior was evoked with a much smaller amount of AVT when the hormone was … More administered intracracially than when given intaperitoneally. Involvement of endogenous AVT in spontaneously occurring courtship behavior was evidenced by the fact that administration of a V1 (vasopressor) receptor antagonist suppressed the expression of the courtship behavior. The water in which AVT-treated males had been kept showed considerable female-attracting activity. Moreover, the content of sodefrin was decreased by the intraperitoneal injection of AVT, suggesting that the AVT stimulated the release of sodefrin from the abdominal gland into the water. AVT induced contraction of the excised abdominal gland concentration-dependently ; and the V1 receptor antagonist suppressed the AVT-induced contraction. Thus, we concluded that AVT induces the pheromone discharge, acting peripherally on a contractile structure of the abdominal gland. AVT was also found to induce spermatophore deposition in the male. Administration of the V1 receptor blocker, to the males suppressed the spermatophore deposition. All these results indicate, the involvement of AVT in reproductive events acting centrally and peripherally. Less

1.蝾螈的雌性吸引信息素“sodefrin”被认为是通过雌性的嗅觉器官起作用的，因为通过双侧鼻孔堵塞或鼻腔和嗅球之间的神经横断可以消除对该物质的吸引力。电生理学研究表明，当将 sodefrin 应用于女性犁鼻上皮的中央区域时，会引起明显的嗅觉电图 (EOG) 反应。已确定犁鼻上皮中犁鼻受体细胞的轴突终止于副嗅球。我们通过将sodefrin应用于女性犁鼻区，观察了副嗅球附近区域的各种电生理反应。2.精氨酸催产素(AVT)对男性生殖事件的影响。 AVT 增强了雄激素诱导的求偶行为的表达。 AVT 可能会发挥中枢作用，因为当激素在颅内施用时，与腹膜内施用相比，用更少量的 AVT 即可诱发行为。内源性 AVT 参与自发发生的求爱行为可以通过施用 V1（血管加压药）受体拮抗剂抑制求偶行为的表达这一事实得到证明。饲养经过 AVT 处理的雄性的水显示出相当大的雌性吸引活性。此外，腹腔注射AVT可降低sodefrin的含量，表明AVT刺激sodefrin从腹腺释放到水中。 AVT 诱导切除的腹部腺体浓度依赖性收缩； V1受体拮抗剂抑制了AVT诱导的收缩。因此，我们得出结论，AVT 诱导信息素释放，作用于腹部腺体的收缩结构。 AVT 还被发现会诱导男性精囊沉积。给雄性注射 V1 受体阻滞剂可抑制精子沉积。所有这些结果表明，AVT 在生殖事件中的作用是中枢性和外周性的。较少的