Laser Micromanipulation Using Microtools at Micro Fluidic Environment

(一)微工具的设计与制作(1)粘附性微工具的利用：我们成功地将酵母细胞附着并在没有直接激光照射的情况下将其输送到靶细胞。(2)微工具的制作：利用光固化树脂，我们通过组装微珠制作了几种微工具，如荧光微工具、彩色微工具和功能微工具。(3)利用微工具操纵微生物：在微工具上固定了一种微生物，(Ii)微工具的制备与环境控制(1)微工具的制备方法：通过调节光固化树脂的密度和紫外光的照射，在芯片内部制作微工具。(2)微流体环境的设计与制作：制作了微芯片，并将细胞固定在芯片中。控制流体环境以测量细胞反应。我们完成了…更多的酵母细胞培养和荧光反应实验。微通道内流体状态的控制：我们设计并制作了一种利用热敏水凝胶加热凝胶化的微牛犊。微通道内的流动状态易于控制。(3)利用微工具实现目标物体的位姿稳定控制(1)激光微操作：推导出多个微工具沿每个给定轨迹的稳定条件。分析中考虑了布朗运动的影响。稳定地实现了多目标的轨迹控制。我们还测量了光陷力。通过实验验证了所提出的轨迹控制方法的有效性。(2)激光微操作的位姿控制：我们组装了三个三角形的微珠，并独立控制其位姿。较少

(I)Design and Fabrication of Microtools(1)Utilization of adherent microtools : We adhered the yeast cell and succeeded in transportation of it without direct laser irradiation to the target cell.(2)Fabrication of microtools : Using photoclosslinkable resin, we made several microtools, such as fluorescent microtool, colored microtool, and functional microtools by assembling microbeads.(3)Manipulation of microorganism using microtools : A microorganism was immobilized on the microtool, and was manipulated indirectly by trapping the microtool made of photoclosslinkable resin in high speed.(II)Preparation of Microtools and Environmental Control(1)Method of preparation of microtools : We fabricated microtools inside the chip by adjusting the density of photoclosslinkable resin and irradiation of UV light.(2)Design and fabrication of micro fluidic environment : We made a micro-chip and immobilized cells in it. The fluid environment was controlled for measurement of cell reaction. We performe … More d experiments of culture and fluorescent reaction of yeast cells. A single cell analysis was possible with this technology.(3)Control of fluid condition in the micro-channel : We designed and fabricated a micro-calve using thermo sensitive hydro gel, which is gelled by heating. The flow condition in the micro-channel was controlled easily.(III)Stabilization of Position and Orientation Control of Target Object Using Microtools(1)Stable manipulation by laser micromanipulation : We derived stability condition of the multiple microtools along each given trajectories. Effect of the Brownian motion was considered in the analysis. We succeeded in trajectory control of multiple targets stably. We also measured the optical trap force. Effectiveness of the proposed trajectory control method was confirmed through the experiments.(2)Position and orientation control by laser micromanipulation : We assembled three microbead in triangular shape and controlled its position and orientation independently. Less