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Laser Micromanipulation Using Microtools at Micro Fluidic Environment

在微流体环境下使用微型工具进行激光显微操作

基本信息

批准号：
14550233
负责人：
ARAI Fumihito
金额：
$ 2.24万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

(I)Design and Fabrication of Microtools(1)Utilization of adherent microtools : We adhered the yeast cell and succeeded in transportation of it without direct laser irradiation to the target cell.(2)Fabrication of microtools : Using photoclosslinkable resin, we made several microtools, such as fluorescent microtool, colored microtool, and functional microtools by assembling microbeads.(3)Manipulation of microorganism using microtools : A microorganism was immobilized on the microtool, and was manipulated indirectly by trapping the microtool made of photoclosslinkable resin in high speed.(II)Preparation of Microtools and Environmental Control(1)Method of preparation of microtools : We fabricated microtools inside the chip by adjusting the density of photoclosslinkable resin and irradiation of UV light.(2)Design and fabrication of micro fluidic environment : We made a micro-chip and immobilized cells in it. The fluid environment was controlled for measurement of cell reaction. We performe … More d experiments of culture and fluorescent reaction of yeast cells. A single cell analysis was possible with this technology.(3)Control of fluid condition in the micro-channel : We designed and fabricated a micro-calve using thermo sensitive hydro gel, which is gelled by heating. The flow condition in the micro-channel was controlled easily.(III)Stabilization of Position and Orientation Control of Target Object Using Microtools(1)Stable manipulation by laser micromanipulation : We derived stability condition of the multiple microtools along each given trajectories. Effect of the Brownian motion was considered in the analysis. We succeeded in trajectory control of multiple targets stably. We also measured the optical trap force. Effectiveness of the proposed trajectory control method was confirmed through the experiments.(2)Position and orientation control by laser micromanipulation : We assembled three microbead in triangular shape and controlled its position and orientation independently. Less

（I）微工具的设计和制造（1）粘附微工具的利用：我们粘附酵母细胞并成功地将其运输到靶细胞而不直接激光照射。（2）微工具的制备：利用光交联树脂，通过组装微珠，制备了荧光微工具、彩色微工具和功能微工具。（3）使用微工具的微生物操作：将微生物固定在微工具上，并通过高速捕获由光交联树脂制成的微工具来间接操作微生物。（II）微工具的制备和环境控制（1）微工具的制备方法：我们通过调节光交联树脂的密度和UV光的照射来在芯片内部制造微工具。（2）微流控环境的设计与制作：制作了微流控芯片，并将细胞固定在芯片中，控制微流控环境，实现细胞反应的检测。我们执行 ...更多信息酵母细胞的培养和荧光反应实验。单细胞分析是可能的，用这种技术。（3）微通道内流体状态的控制：设计并制作了一种采用热敏水凝胶的微通道。微通道内的流动状态易于控制。（III）使用微工具的目标对象的位置和取向控制的稳定性（1）通过激光微操纵的稳定操纵：我们推导了多个微工具沿着每个给定轨迹的稳定性条件。分析中考虑了布朗运动的影响。成功实现了多目标稳定的弹道控制。我们还测量了光阱力。通过实验验证了所提出的轨迹控制方法的有效性。（2）激光微操作的位置和取向控制：我们将三个微珠组装成三角形，并独立控制其位置和取向。少