We propose to analyze how membrane trafficking and the tissue polarity proteins control junctional remodeling and prepupal wing morphogenesis

我们建议分析膜运输和组织极性蛋白如何控制连接重塑和预蛹翅形态发生

• 批准号: 5331244
• 负责人: Professorin Dr. Suzanne Eaton (†)
• 金额: --
• 依托单位: Biotechnologisches Zentrum (Biotec)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5331244?language=en
• 关键词: propose; analyze; how; membrane; trafficking

In order to form well-ordered tissues, epithelial cells coordinate the polarity of subcellular structures along an axis within the plane of the epithelium. In vertebrates, ciliated epithelia and the sensory hair cells in the inner ear are just two examples of epithelia in which planar polarization is critical for function. In the Drosophila wing, planar polarity is reflected in the uniform distal orientation of wing hairs, one of which is made by each cell. The planar axis is communicated between cells hours before wing hairs form; the earliest molecular readout of polarity is localization of Flamingo, a homophilic adhesion molecule, to the proximal and distal cell boundaries. Localization depends on Frizzled-mediated intercellular signaling, but the molecular connections between Frizzled signaling and Flamingo localization are not understood. Recently, we have shown that Diego, a protein with six ankyrin repeats, also localizes to proximal-distal boundaries and is required for Frizzled-dependent localization of Flamingo. The experiments we propose are designed to identify the cell biological mechanisms of Diego and Flamingo polarization, and to determine how the direction of their polarization is communicated to other cells.

为了形成有序的组织，上皮细胞沿上皮平面内的轴沿着协调亚细胞结构的极性。在脊椎动物中，纤毛上皮细胞和内耳中的感觉毛细胞只是其中平面极化对功能至关重要的上皮细胞的两个例子。在果蝇翅膀中，平面极性反映在翅膀毛的均匀远端方向上，其中一个由每个细胞组成。在翅毛形成前几个小时，平面轴在细胞之间进行通信;极性的最早分子读数是Flamingo（一种嗜同性粘附分子）在近端和远端细胞边界的定位。定位依赖于卷曲介导的细胞间信号，但卷曲信号和火烈鸟定位之间的分子联系还不清楚。最近，我们已经表明，迭戈，一种蛋白质与六个锚蛋白重复，也定位到近端-远端边界，并需要卷曲依赖本地化的火烈鸟。我们提出的实验旨在确定Diego和Flamingo极化的细胞生物学机制，并确定其极化方向如何与其他细胞沟通。