The role of lipid-raft-associated cell adhesion molecules, tyrosine kinases and associated cytoskeleton in polarity of myelinating oligodendrocytes

脂筏相关细胞粘附分子、酪氨酸激酶和相关细胞骨架在髓鞘少突胶质细胞极性中的作用

• 批准号: 5331300
• 负责人: Professorin Dr. Jacqueline Trotter, Ph.D.
• 金额: --
• 依托单位: Institut für Entwicklungsbiologie und Neurobiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5331300?language=en
• 关键词: role; lipid; raft; associated; cell

The aim of the project is to define the role of lipid-protein complexes or "rafts", which act as sorting and signalling platforms, in the establishment and maintenance of the polarity of myelinating glial cells. We will focus on the interaction between raft-associated adhesion molecules, signalling molecules, and cytoskeletal elements, which are established during initial contact of axons and glial cells. In particular the role of the cytoskeleton associated with oligodendrocyte rafts in inducing process outgrowth necessary for axonal wrapping will be investigated. In addition we will characterise rafts in Schwannn cells (SC) and pay particular attention to the presence of Srcfamily kinases, the L1-cell adhesion molecule, integrins and cytoskeletal components. Finally, we plan to analyse the dynamic behaviour of the cytoskeleton in oligodendrocytes and SC using Enhanced Green Fluorescent Protein-labelled cytoskeletal components and analyse the effect of inhibiting raft-cytoskeleton-association on process outgrowth and axonal contact.

该项目的目的是确定脂质-蛋白质复合物或“筏”的作用，它们作为分选和信号平台，在髓鞘形成神经胶质细胞极性的建立和维持中发挥作用。我们将集中在筏相关的粘附分子，信号分子和细胞骨架元素，这是建立在最初的接触轴突和神经胶质细胞之间的相互作用。特别是与少突胶质细胞筏相关的细胞骨架在诱导轴突包裹所需的过程中的作用将被研究。此外，我们将在Schwannn细胞（SC）中进行标记，并特别注意Src家族激酶，L1细胞粘附分子，整合素和细胞骨架成分的存在。最后，我们计划使用增强型绿色荧光蛋白标记的细胞骨架成分分析少突胶质细胞和SC中细胞骨架的动态行为，并分析抑制筏细胞骨架协会对过程生长和轴突接触的影响。