Addressing the specificity and function of NG2 cell-neuron synaptic contacts: NG2 cells as modulators of the neuronal network?

解决 NG2 细胞-神经元突触接触的特异性和功能：NG2 细胞作为神经元网络的调节剂？

• 批准号: 255070194
• 负责人: Professorin Dr. Jacqueline Trotter, Ph.D.
• 金额: --
• 依托单位: Institut für Entwicklungsbiologie und Neurobiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/255070194?language=en
• 关键词: Addressing; specificity; function; NG2; cell

In this project we will focus on the potential role of NG2 cells in modulating the neuronal network. This will be by examining gene products made by NG2 cells with neuroregulatory function and elucidating their temporal and spatial heterogeneity of expression in this cell population. We will utilise information we already have in hand as well as new candidates isolated by deep sequencing of neuronal-NG2 cell synaptosomes to identify locally translated mRNA. Furthermore, we will examine whether the activity-dependent cleavage of NG2 that we have recently discovered, which releases the ectodomain into the brain extracellular matrix, is a general phenomenon or shows regional variation in the NG2 cell population. We will examine how this affects the neuronal network by electrophysiological analysis of neurons in the somatosensory cortex and hippocampus. In a second complementary part we will identify the origin of synaptic contacts onto NG2 glia by the surrounding neuronal network using a rabies-virus based tracing method and examine its regional and temporal heterogenity and whether this pattern is modulated by physiological stimuli.

在这个项目中，我们将重点关注 NG2 细胞在调节神经元网络中的潜在作用。这将通过检查具有神经调节功能的 NG2 细胞产生的基因产物，并阐明它们在该细胞群中表达的时间和空间异质性。我们将利用现有的信息以及通过神经元 NG2 细胞突触体深度测序分离出的新候选者来识别本地翻译的 mRNA。此外，我们将检查我们最近发现的 NG2 活性依赖性裂解（将胞外域释放到大脑细胞外基质中）是否是 NG2 细胞群中的普遍现象或显示区域差异。我们将通过对体感皮层和海马体神经元的电生理分析来研究这如何影响神经元网络。在第二个补充部分中，我们将使用基于狂犬病病毒的追踪方法来识别周围神经元网络与 NG2 胶质细胞的突触接触的起源，并检查其区域和时间异质性以及这种模式是否受到生理刺激的调节。