Development of functional cell scaffolds and bioreactors for regenerative medicine based on stem cells

本研究的目的是探索和发展生物医学技术和方法，以促进干细胞的增殖和分化，这是实现组织再生治疗所必需的。由不同的生物可降解聚合物设计和制备了各种三维海绵和细胞支架非织造布，同时通过在支架中加入细胞黏附物质来改善和提高支架与细胞的相容性，从而创造出功能支架。此外，将生物反应器技术与功能支架相结合，促进干细胞的体外增殖和分化。结果表明：通过纤维的掺入对支架进行机械增强，利用烷基硫醇的自组装技术对支架进行表面修饰，通过固定化细胞黏附物质来改善支架的细胞相容性，通过将支架与各种生物反应器相结合来促进细胞的体外增殖和分化。人们期待所有的研究成果不仅对基础生物学和医学的研究加速，而且对组织再生疗法的未来发展做出强有力的贡献。

The objective of this research is to investigate and develop biomedical technologies and methodologies for the enhancement of stem cells proliferation and differentiation which is necessary to realize tissue regeneration therapy. Various 3-dimensional sponges and non-woven fabics of cell scaffold have been designed and prepared from different biodegradable polymers while the compatibility of scaffolds to cells is modified and improved by incorporating cell adhesion substances into them to and create functional scaffolds. In addition, bioreactor technologies are combined with the functional scaffold to promote the proliferation and differentiation of stem cells in vitro. The results obtained are as follows: The mechanical reinforcement of scaffolds by the incorporation of fibers, the surface modification of scaffolds with the self-assemble technology of alkanethiols, the improvement of scaffold cytocompatibility by the immobilization of cell adhesion substances, the promotion of cells proliferation and differentiation in vitro by combining the scaffold with various bioreactors. It is highly expected that all the results strongly contribute not only to the research acceleration of basic biology and medicine, but also the future development of tissue regeneration therapy.