Development of Technology to Manipulate the Biological Functions of Stem Cells for Cell Therapy of Next Generation

将精胺化学引入明胶中，制备了基因转染用阳离子明胶。通过陶瓷颗粒掺入机械增强的明胶海绵支架与生长因子和钙化明胶和质粒DNA复合物结合。骨髓间充质干细胞（MSC）在制备的海绵中进行基因转染。与常规基因转染相比，MSC基因工程具有更高的效率。与培养基循环系统的生物反应器相结合，进一步提高了工程效率。该培养技术适用于小干扰DNA，是一种很有前途的细胞生物活性工程技术。

A cationized gelatin of gene transfection was prepared by chemical introducing spermine to gelatin. Gelatin sponge scaffolds mechanically reinforced by ceramic granules incorporation were combined with growth factors and a complex of ca tionized gelatin and plasmid DNA. Bone marrow derived mesenchymal stem cells (MSC) were cultured for gene transfection in the sponge prepared. Comparing with the conventional gene transfection, a higher efficiency of MSC genetically engineering was observed. Upone combining with a bioreactor of culture medium circulating system, the engineering efficiency was further improved. This culture technology is applicable for small interfering DNA, and demonstrated to be a promising engineering technology of cell bioactivity.