Biomaterials incorporating releasable growth factor

含有可释放生长因子的生物材料

• 批准号: 06680854
• 负责人: TABATA Yasuhiko
• 金额: $ 1.47万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06680854/
• 关键词: Growth factor; Gelatin; Hydrogel; Release; Neovascularization; Biodegradation; bFGF

Basic fibroblast growth factor (bFGF) was impregnated through polyin complexation into biodegradable hydrogels prepared by crosslinking of acidic gelatin with the isoelectric point of 4.9. Release experiments demonstrated not to release bFGF from the gelatin hydrogel under the in vitro non-degradation conditions. The hydrogels were degraded with time following their subcutaneous implantation on the mouse back and the period of in vivo degradation varied depending on the water content of hydrogels. The gelatin hydrogels containing bFGF strongly induced neovascularization around the implantation site, whereas subcutaneous injection of bFGF in the solution form did not increase the amount of tissue hemoglobin and the wet weight of tissue. The bFGF-impregnated gelatin hydrogel of lower water content retained the vascularization effect for a longer period. It was concluded that bFGF was released from gelatin hydrogel as a result of their in vivo degradation, leading to more efficient induction of neovascularization than free bFGF without impregnation in the hydrogel.

以等电点为4.9的酸性明胶为交联剂，通过多聚体络合将碱性成纤维细胞生长因子浸渍到生物可降解水凝胶中。体外释放实验表明，在体外非降解条件下，明胶水凝胶不能释放碱性成纤维细胞生长因子。这些水凝胶在小鼠背部皮下植入后会随着时间的推移而降解，体内降解的时间取决于水凝胶的含水量。含有碱性成纤维细胞生长因子的明胶水凝胶强烈诱导植入部位周围的新生血管，而皮下注射碱性成纤维细胞生长因子溶液并不增加组织中的血红蛋白含量和组织湿重。较低含水量的碱性成纤维细胞生长因子明胶水凝胶具有较长时间的血管生成作用。结论：碱性成纤维细胞生长因子在体内降解后从明胶水凝胶中释放出来，比未浸渍的游离碱性成纤维细胞生长因子更有效地诱导新生血管形成。

项目成果

Yasuhiko Tabata: "Enhancedvascularization and tissue granulation by basic fibroblastgrowth factor impregnated in gelatin hydrogels" J.Controlled Release. 13. 189-199 (1994)

Yasuhiko Tabata：“通过明胶水凝胶中浸渍的碱性成纤维细胞生长因子增强血管化和组织颗粒化”J.Controlled Release。

Y. Tabata and Y. Ikada: "Potentiated in vivo biological activity of basic fibroblast growth factor by incorporation into polymer hydrogel microsphere" Proceedings of 4th Japan International SAMPE Symposium. 4. 577-582 (1995)

Y. Tabata 和 Y. Ikada：“通过纳入聚合物水凝胶微球增强碱性成纤维细胞生长因子的体内生物活性”第四届日本国际 SAMPE 研讨会论文集。