Genetic factor and alternation of physical performance.-Angiotensin I converting enzyme gene polymorphism and phenotypes-

遗传因素与身体机能的交替。-血管紧张素I转换酶基因多态性和表型-

• 批准号: 15300229
• 负责人: KIYONAGA Akira
• 金额: $ 10.3万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300229/
• 关键词: ACE; SNP; Exercise; Hormone; Aging; Strength; Adaptation; QTc; アンジオテンシンI変換酵素; 遺伝子多型; 運動トレーニング; ノックアウトマウス; 高齢者; 遺伝子; 循環動態

In this project, follow 3 investigations were carried out. (1)Whether angiotensin I converting enzyme (ACE) gene insertion/deletion (I/D) Polymorphism influence hormonal response and blood pressure during a single bout of exercise. (2)Relationship between ACE gene I/D polymorphism and strength decline with aging. (3)Whether ACE gene I/D polymorphism affect the effects of exercise training in elderly.(1)Twelve young men with I/I or D/D of ACE gone I/D polymorphism were participated this study. They performed moderate, middle and high intensity exercise test. Surprisingly, serum angiotensin (Ang) II concentration was not difference at rest, during and after exercise between the genotypes. Ang II response depend on Ang I, which is substrate of Ang II. The results suggest that the ACE gene I/D polymorphism is not strong factor of Ang II production.(2)One hundred ninety elderly people were participated in this study. The leg extension power, knee extension strength and grip strength were evaluated for 4 years. As the results, there indexes are similar change among genotypes. This results suggest that strength decline with aging is not influenced by ACE gene I/D polymorphism.(3)One hundred thirty five elderly were participated in this study. They performed bench step exercise training for 12weeks at the lactate threshold intensity. As the results, the training responses of anaerobic capacity and leg extension power were similar among the genotypes. Interestingly, corrected QT, which is index of heart function, is improved in only women with D/D.There results suggest that however, the ACE gene I/D polymorphism is not strongly genetic factor to determine human physical performance, the mechanism of the adaptation to exercise training is influenced the gene polymorphism.

在本项目中，进行了以下3项调查。（1）血管紧张素转换酶（ACE）基因插入/缺失（I/D）多态性是否影响单次运动时的激素反应和血压。（2）ACE基因I/D多态性与增龄性力量衰退的关系。（3）ACE基因I/D多态性是否影响老年人运动训练的效果。（1）12名ACE基因I/D多态性为I/I或D/D的男性青年参与本研究。分别进行中、中、高强度运动试验。令人惊讶的是，血清血管紧张素（Ang）II浓度在休息时，运动期间和运动后的基因型之间没有差异。Ang II的反应依赖于Ang I，而Ang I是Ang II的底物。提示ACE基因I/D多态性不是Ang Ⅱ产生的强有力因素。（2）190名老年人参加了本研究。随访4年，评价下肢伸展力、膝关节伸展力和握力。结果表明，各基因型之间的指标变化趋势相似。这一结果表明，随着年龄的增长，力量下降是不受ACE基因I/D多态性。（3）135名老年人参加了本研究。在乳酸阈强度下进行12周的台阶运动训练。结果表明，不同基因型的无氧能力和腿部伸展力量的训练反应相似。有趣的是，作为心功能指标的校正QT，仅在D/D女性中得到改善。这些结果表明，ACE基因I/D多态性不是决定人类运动能力的强遗传因素，基因多态性影响运动训练的适应机制。

项目成果

Association of Angiotensin I Converting Enzyme Gene Insertion/Deletion Polymorphism with the Renin-Angiotensin System and Blood Pressure Response during a Single Bout of Exercise.

血管紧张素 I 转换酶基因插入/缺失多态性与肾素-血管紧张素系统和单次运动期间血压反应的关联。

• 期刊: International Journal of Sport and Health Science (In press)
• 作者: Takuro Tobina;Akira Kiyonaga;et al.
• 通讯作者: et al.

Simple and rapid detection of uncoupling protein-2-866G/A polymorphism by mutagenically separated polymerase chain reaction

通过诱变分离聚合酶链式反应简单快速地检测解偶联蛋白-2-866G/A 多态性

• 发表时间: 2004
• 期刊: Clinica Chimica Acta. 344
• 作者: Bo Zhang;Hiroaki Tanaka;Keijiro Saku
• 通讯作者: Keijiro Saku

Relationship between angiotensin converting enzyme gene I/D polymorphism and muscle strength in elderly

血管紧张素转换酶基因I/D多态性与老年人肌力的关系

• 发表时间: 2006
• 期刊: Int J Sport and Health Science 4
• 作者: Takuro Tobina;Makoto Ayabe;Yutaka Yoshitake;Yasuo Kimura;Hideo Miyazaki;Kojiro Ishii;Bo Zhang;Keijiro Saku;Munehiro Shindo;Akira Kiyonaga and Hiroaki Tanaka
• 通讯作者: Akira Kiyonaga and Hiroaki Tanaka

Simple and rapid detection of uncoupling protein-2 -866G/A Polymorphism by mutagenically separated polymerase chain reaction

通过诱变分离聚合酶链式反应简单快速地检测解偶联蛋白-2 -866G/A 多态性

• 发表时间: 2004
• 期刊: Clinica Chimica Acta 344
• 作者: Bo Zhang;Hiroaki Tanaak et al.
• 通讯作者: Hiroaki Tanaak et al.

Relationship between angiotensin converting enzyme gene I/D polymorphism and muscle strength in elderly.

血管紧张素转换酶基因I/D多态性与老年人肌力的关系

• 发表时间: 2006
• 期刊: Int J Sport and Health Science 4
• 作者: Takuro Tobina;et al.
• 通讯作者: et al.