Magnetic immunoassay system utilizing magnetic marker and SQUID sensor

利用磁标记和 SQUID 传感器的磁免疫分析系统

基本信息

  • 批准号:
    15360220
  • 负责人:
  • 金额:
    $ 9.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2005
  • 项目状态:
    已结题

项目摘要

Immunoassays are widely used to detect disease-related proteins for medical diagnostics. These proteins are generically called antigens, and the so-called binding reaction between antigen and its antibody is used for the immunoassay. In order to improve the performance of the immunoassay, we have developed a new detection system utilizing a magnetic marker and a SQUID sensor. The main results obtained in this study are as follows.(1)We clarified the dependence of the magnetic properties on the size of the magnetic nanoparticle. We showed that the particle with nominal diameter of 25 nm can keep remanence, and can generate large magnetic signal. This particle is, therefore, suitable for magnetic immunoassay.(2)We developed a coating process to cover the magnetic particle with polymer. With this process, we fabricated a polymer-coated magnetic nanoparticle that shows good dispersion in liquid.(3)We developed the high Tc SQUID system for detecting magnetic signal from the marker. Since the signal decays rapidly in space, we realized a close distance between the cooled SQUID and the room temperature sample.(4)We developed a magnetic-contamination free reaction chamber and a method to cancel environmental noise in order to detect very weak signal. Our system can detect 12 samples in a short measurement time of a few minutes with a field resolution of about 10 pT.(5)We conducted a detection of protein called IgE. We demonstrated the detection of IgE down to 0.3 pg, which shows the high sensitivity of the present magnetic method. We also demonstrated the detection of IgE in human serum, which shows the possibility to practical diagnosis.
免疫测定广泛用于检测疾病相关蛋白质以用于医学诊断。这些蛋白质统称为抗原,抗原与其抗体之间的所谓结合反应用于免疫测定。为了提高免疫分析的性能,我们开发了一种新的检测系统,利用磁性标记和SQUID传感器。本研究的主要结果如下。(1)We阐明了磁性纳米颗粒的大小对磁性的依赖性。结果表明,标称粒径为25 nm的纳米粒子能够保持剩磁,并产生较大的磁信号。因此,该颗粒适用于磁性免疫测定。(2)We开发了一种涂层工艺,用聚合物覆盖磁性颗粒。利用这一过程,我们制备了一种聚合物包覆的磁性纳米颗粒,在液体中表现出良好的分散性。(3)We开发了用于检测来自标记的磁信号的高Tc SQUID系统。由于信号在空间中衰减很快,我们实现了冷却SQUID和室温样品之间的近距离。(4)We研制了一种无磁污染的反应室和消除环境噪声的方法,以检测非常微弱的信号。我们的系统可以在几分钟的短测量时间内检测12个样品,场分辨率约为10 pT。(5)We进行了一项名为IgE的蛋白质检测。我们证明了IgE的检测低至0.3 pg,这表明本磁性方法的高灵敏度。我们还证明了检测人血清中的IgE,这表明实际诊断的可能性。

项目成果

期刊论文数量(43)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Design of superconducting quantum interference device for magnetic immunoassays
磁免疫分析超导量子干涉装置的设计
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    栗原;清水;小野;安藤;K.Enpuku et al.;K.Enpuku;K.Enpuku et al.;T.Q.Yang et al.;K.Enpuku et al.;T.Q.Yang et al.;T.Q.Yang et al.;K.Enpuku et al.
  • 通讯作者:
    K.Enpuku et al.
T.Q.Yang et al.: "SQUED magnetometer utilizing normal pickup coil and resonant-type coupling circuit"Physica C. 392-396. 1396-1400 (2003)
T.Q.Yang 等人:“利用普通拾波线圈和谐振型耦合电路的 SQUED 磁力计”Physica C. 392-396。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Magnetic immunoassays utilizing magnetic markers and a high-TC SQUID
Quantitative eveluation of magnetic immunoassay with remanence measurement
剩磁测量磁免疫分析的定量评价
円福 敬二: "SQUIDを用いたバイオ免疫検査"低温工学. Vol.39No.9. 17-24 (2003)
Keiji Enpuku:“使用 SQUID 进行生物免疫测试”Cryogenic Engineering Vol.39No.9 (2003)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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ENPUKU Keiji其他文献

ENPUKU Keiji的其他文献

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{{ truncateString('ENPUKU Keiji', 18)}}的其他基金

Magnetic-field assisted binding method between magnetic markers and targets for biomedical applications
生物医学应用中磁性标记物与靶标之间的磁场辅助结合方法
  • 批准号:
    16K14277
  • 财政年份:
    2016
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
SQUID pico-voltmeter for highly sensitive measurement system
用于高灵敏度测量系统的 SQUID 皮伏表
  • 批准号:
    22656093
  • 财政年份:
    2010
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Liquid-phase detection of biological targets with magnetic markers
利用磁性标记对生物目标进行液相检测
  • 批准号:
    21360199
  • 财政年份:
    2009
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Magnetic Immunoassay system without BF separation for rapid and sensitive detection of biological targets
无需 BF 分离的磁免疫分析系统,可快速、灵敏地检测生物靶标
  • 批准号:
    18360199
  • 财政年份:
    2006
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
SQUID Microscope System for Biological Immunoassay
用于生物免疫分析的 SQUID 显微镜系统
  • 批准号:
    12555117
  • 财政年份:
    2000
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of high-frequency current probe utilizing high T_c Josephson junction
利用高T_c约瑟夫森结的高频电流探头的开发
  • 批准号:
    12650430
  • 财政年份:
    2000
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of high Tc SQUID magnetometer system for biological application
生物应用高Tc SQUID磁力计系统的开发
  • 批准号:
    10650412
  • 财政年份:
    1998
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of high Tc SQUID microscope for biological application
生物应用高Tc SQUID显微镜的开发
  • 批准号:
    09555128
  • 财政年份:
    1997
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of High T_C SQUID Magnetometer Systm
高T_C SQUID磁力计系统的研制
  • 批准号:
    08650496
  • 财政年份:
    1996
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of High Tc SQUID Magnetometer
高温SQUID磁力计的研制
  • 批准号:
    06650472
  • 财政年份:
    1994
  • 资助金额:
    $ 9.41万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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调整脉冲激光退火技术开发重掺杂Q-碳高温超导体
  • 批准号:
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    15K04688
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p电子体系高温超导体的研制
  • 批准号:
    24684028
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高温超导/铁磁体混合体中的量子输运及其在量子比特中的应用
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高温超导体赝能隙态折叠费米面的观察
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    22740221
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由Sr、Cu和O组成的高温超导体的合成以及通过顶端O原子的有序化提高Tc
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利用高温超导磁体控制高温超导带中的电磁现象以产生稳定均匀的高磁场
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Fluxon Dynamics of High-TC superconductor and device application for THz wave.
高TC超导体Fluxon动力学及太赫兹波器件应用。
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    20360151
  • 财政年份:
    2008
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高温超导体纳米结构中的量子输运和自旋控制
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  • 资助金额:
    $ 9.41万
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