权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of high Tc SQUID microscope for biological application

生物应用高Tc SQUID显微镜的开发

基本信息

批准号：
09555128
负责人：
ENPUKU Keiji
金额：
$ 8.32万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09555128/
关键词：
SQUID magnetometer SQUID microscope magnetic nanoparticles immunoassays magnetic marker high Tc superconductor 1 f noise 30-degree bicrystal junction SQUID 磁気センサジョセフソン素子バイクリスタル基板ノイズ磁束ノイズ

项目摘要

In this research, we developed high Tc SQUID magnetic-microscope system for biological application. Our results are summarized as follows.1. We developed high performance SQUID utilizing bicrystal junctions with 30-degree misorientation angles. At 77 K, we can routinely obtain the voltage modulation depth ΔV>20μV and the flux noise SィイD2φィエD2ィイD11/2ィエD1<12μφィイD2oィエD2/HzィイD11/2ィエD1 for 100pH-SQUID.2. We significantly reduced the 1/f noise of the direct-coupled magnetometer. In order to avoid the flux trapping in the pickup coil, we develop a pickup coil which consists of 4 parallel loop with narrow line-width and involves the so-called flux dam. With this pickup coil, the flux noise is reduced to SィイD2φィエD2ィイD11/2ィエD1=23μφィイD2oィエD2/HzィイD11/2ィエD1 at f=1 Hz. This values is only 2.3 times higher than the white noise of SィイD2φィエD2ィイD11/2ィエD1=10μφo/HzィイD11/2ィエD1.3. We developed a SQUID microscope system in order to detect FeィイD22ィエD2OィイD23ィエD2 magnetic nanoparticles. A special Dewar to make a distance between the SQUID and sample as small as 1 mm is developed. The system works well even when the magnetic field of 10 Gauss is applied in parallel to the SQUID.4. Biological immunoassay is performed with the present system. In the present system, as antibody is labeled with FeィイD22ィエD2OィイD23ィエD2 nanoparticles. Binding reaction between antigen and its antibody can be detected by measuring the magnetic field from FeィイD22ィエD2OィイD23ィエD2 nanoparticles. It is shown that the present system is 10 times more sensitive than the conventional method using an optical marker. It will be possible to improve the sensitivity by more than factor 10.

在本研究中，我们发展了一套生物应用的高温超导量子干涉仪磁显微系统。主要研究结果如下：1.我们开发了高性能SQUID利用双晶体结与30度取向角。在77 K下，我们可以常规地获得100 pH-SQUID的电压调制深度ΔV>20μV和磁通噪声S_（？）D_（？）我们显著降低了直接耦合磁强计的1/f噪声。为了避免拾取线圈中的磁通陷阱，我们开发了一种拾取线圈，它由4个窄线宽的平行回路组成，并涉及所谓的磁通坝。利用该拾波线圈，在f=1 Hz时，磁通噪声降低到S D2 φ D2 D11/2 D1=23μφ D2 o D2/Hz D11/2 D1。该值仅比S D 2 φ D 2 D 11/2 D 1 =10μφo/Hz D 11/2 D 1.3的白色噪声高2.3倍。我们开发了一个SQUID显微镜系统，以检测Fe D22 D2 O D23 D2磁性纳米颗粒。研制了一种特殊的杜瓦瓶，使SQUID与样品之间的距离小至1 mm。即使在10高斯的磁场平行于SQUID的情况下，系统也能很好地工作。用本系统进行生物免疫测定。在本发明的系统中，抗体是用Fe 2 + D22 + D2 O + D23 + D2纳米颗粒标记的。抗原与其抗体之间的结合反应可以通过测量来自Fe纳米颗粒D22纳米颗粒D23纳米颗粒D22纳米颗粒D2 O纳米颗粒D23纳米颗粒D2纳米颗粒的磁场来检测。它表明，本系统是10倍以上的灵敏度比传统的方法，使用光学标记。将有可能将灵敏度提高10倍以上。