Isolation of Denitrifying Phosphorus Removing Bacteria and Application of the Biochemical Information for Optimal Control of Advanced Wastewater Treatment Plant

提出了一种全新的筛选装置，用于分离高效聚磷菌。在厌氧-缺氧交替条件下进行了筛选工作。仅在厌氧阶段，乙酸盐被用作唯一的有机质。在缺氧阶段，硝酸盐作为最终的电子受体，不添加有机物。由于在从一种条件到另一种条件的转换阶段用蒸馏水清洗设备内部，因此在工作期间乙酸盐和硝酸盐不会同时存在。作为在城市污水处理厂中使用活性污泥的这些工作的结果，获得了几种细菌。几乎孤立的细菌能够积累聚磷酸盐和聚羟基丁酸酯在独特的条件下，如交替厌氧-缺氧条件。通过染色法显微镜观察，可以合理地定性地假设这些细菌负责增强生物除磷。然而，没有定量观察到这些纯培养物在厌氧条件下释放磷酸盐和乙酸盐，在缺氧条件下消耗磷酸盐。

A brand-new screening equipment was proposed to isolate the denitrifying phosphate-accumulating organisms. A screening work was carried out under the alternating anaerobic-anoxic condition. At only anaerobic phase, acetate was used as a sole organic matter. At the anoxic phase, nitrate as a final electron acceptor and no organic matters were added. Since the inside of equipment was washed with distilled water at the shifting phase from one condition to another condition, acetate and nitrate did not exist simultaneously during the work. As a result of these works using activated sludge in a municipal waste water treatment plant, several kinds of bacteria were obtained. Almost isolated bacteria were able to accumulate polyphosphate and PHB under unique conditions such as alternating anaerobic-anoxic conditions. By microscopy with a staining method it was reasonable to suppose qualitatively that these bacteria were responsible for enhanced biological phosphorus removal. However, it was not quantitatively observed that these pure cultures released phosphate with acetate under anaerobic conditions and consumed phosphate under anoxic conditions.