Isolation of Denitrifying Phosphorus Removing Bacteria and Application of the Biochemical Information for Optimal Control of Advanced Wastewater Treatment Plant

提出了一种用于反硝化聚磷菌分离的新型筛选设备。在厌氧-缺氧交替条件下进行了筛选工作。仅在厌氧阶段，醋酸盐是唯一的有机物。在缺氧阶段，硝酸盐作为最终的电子受体，不添加有机物。由于设备内部是在从一种状态转换到另一种状态时用蒸馏水清洗的，因此在工作过程中不会同时存在醋酸盐和硝酸盐。通过对某城市污水处理厂活性污泥法的研究，得到了几种细菌。几乎分离的细菌都能在独特的条件下积累聚磷酸盐和PHB，如厌氧-缺氧交替条件。通过显微镜结合染色的方法，定性地推测这些细菌对强化生物除磷负有责任。然而，没有定量观察到这些纯培养物在厌氧条件下与醋酸盐一起释放磷酸盐，在缺氧条件下消耗磷酸盐。

A brand-new screening equipment was proposed to isolate the denitrifying phosphate-accumulating organisms. A screening work was carried out under the alternating anaerobic-anoxic condition. At only anaerobic phase, acetate was used as a sole organic matter. At the anoxic phase, nitrate as a final electron acceptor and no organic matters were added. Since the inside of equipment was washed with distilled water at the shifting phase from one condition to another condition, acetate and nitrate did not exist simultaneously during the work. As a result of these works using activated sludge in a municipal waste water treatment plant, several kinds of bacteria were obtained. Almost isolated bacteria were able to accumulate polyphosphate and PHB under unique conditions such as alternating anaerobic-anoxic conditions. By microscopy with a staining method it was reasonable to suppose qualitatively that these bacteria were responsible for enhanced biological phosphorus removal. However, it was not quantitatively observed that these pure cultures released phosphate with acetate under anaerobic conditions and consumed phosphate under anoxic conditions.