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Isolation of Denitrifying Phosphorus Removing Bacteria and Application of the Biochemical Information for Optimal Control of Advanced Wastewater Treatment Plant

反硝化除磷细菌的分离及生化信息在深度污水处理厂优化控制中的应用

基本信息

批准号：
15360288
负责人：
KUBA Takahiro
金额：
$ 9.73万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15360288/
关键词：
Denitrification Dephosphatation DN-PAO Isolation Advanced wastewater treatment Water quality Acetate ASM

项目摘要

A brand-new screening equipment was proposed to isolate the denitrifying phosphate-accumulating organisms. A screening work was carried out under the alternating anaerobic-anoxic condition. At only anaerobic phase, acetate was used as a sole organic matter. At the anoxic phase, nitrate as a final electron acceptor and no organic matters were added. Since the inside of equipment was washed with distilled water at the shifting phase from one condition to another condition, acetate and nitrate did not exist simultaneously during the work. As a result of these works using activated sludge in a municipal waste water treatment plant, several kinds of bacteria were obtained. Almost isolated bacteria were able to accumulate polyphosphate and PHB under unique conditions such as alternating anaerobic-anoxic conditions. By microscopy with a staining method it was reasonable to suppose qualitatively that these bacteria were responsible for enhanced biological phosphorus removal. However, it was not quantitatively observed that these pure cultures released phosphate with acetate under anaerobic conditions and consumed phosphate under anoxic conditions.

提出了一种全新的筛选设备来分离反硝化聚磷微生物。在厌氧-缺氧交替条件下进行筛选工作。仅在厌氧阶段，乙酸盐被用作唯一的有机物。在缺氧阶段，硝酸盐作为最终电子受体，不添加有机物。由于在从一种条件到另一种条件的转换阶段用蒸馏水清洗设备内部，因此在工作过程中乙酸盐和硝酸盐不会同时存在。通过在城市污水处理厂中使用活性污泥进行这些工作，获得了多种细菌。几乎孤立的细菌能够在独特的条件下（例如交替厌氧缺氧条件下）积累多磷酸盐和 PHB。通过染色方法的显微镜检查，可以合理地定性地假设这些细菌负责增强生物磷去除。然而，没有定量观察到这些纯培养物在厌氧条件下释放磷酸盐和乙酸盐，并在缺氧条件下消耗磷酸盐。

项目成果

期刊论文数量（21）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

浜田康治, et al.: "嫌気-無酸素連続回分スクリーニング法による脱窒脱リン細菌単離の試み"環境工学研究論文集. 40. 53-61 (2003)

Koji Hamada 等：“尝试使用厌氧缺氧连续批量筛选方法分离反硝化和脱磷细菌”环境工程研究杂志 40. 53-61 (2003)。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Comparison of Nutrient Removal Efficiency between Pre-and Post-Denitrification Wastewater Treatment

反硝化废水处理前后的营养物去除效率比较

DOI：
发表时间：
2005
期刊：
Proceedings of the International Water Association (IWA) Conference 2005,Xi'an
影响因子：
0
作者：
Vladimir TORRICO et al.;Vladimir TORRICO et al.;Vladimir TORRICO et al.;Koji HAMADA et al.
通讯作者：
Koji HAMADA et al.

脱窒性脱リン細菌の集積と単離に関する検討

反硝化除磷菌的富集与分离研究