Studies on functional domains essential for the retrotransposition of LINE

在项目期间，我们主要使用端粒特异系SART1，阐明了LINE逆转座的必要结构域，如下所示(1到3，TRAS1用于4，R1用于5)。我们在SART1(MatSumoto，T.，et al.Mol.Cell Biol)的ORF1蛋白中发现了新的NLS和端粒加入信号。24,105-122。(2004))。我们在SART1(Kojima，KK.et al.Mol.Cell.Biol.25,7675-7686)中发现了真核翻译偶联对ORF1和ORF2翻译的调控。(2005))。我们阐明了SART1(Osanai，M.等人，Mol.Cell Biol.24,7902-7913)的ReversetrTranscription单元识别的3‘UTR结构。(2004))。与九州大学的Maita博士合作，我们首次报道了TRAS1的内切酶结构域的晶体结构(Maita，N.，et al.J.Biol.Chem.279,41067-41076)。5.我们建立了28SrDNA特异系R1的逆转录检测系统，该系统使我们能够使用几个靶标特异系、端粒特异系SART1和TRAS1以及rDNA特异系R1用于未来的分析(Anzai T.等人，核酸研究结果33,1993-2002)。(2005))。

During the project term, we clarified the essential domains for the retrotranspositions of LINE, using mainly telomere specific LINE SART1, as shown below (1 to 3, TRAS1 for 4, R1 for 5).1. We found novel domains NLS and telomere accession signals in ORF1 proteins in SART1 (Matsumoto, T., et al.Mol.Cell Biol. 24,105-122. (2004)).2. We found eukaryotic translational coupling for the regulation of ORF1 and ORF2 translation in SART1 (Kojima, KK.et al.Mol.Cell.Biol.25, 7675-7686. (2005)).3. We clarified the 3'UTR structure which is recognized by reversetranscription unit of SART1 (Osanai, M., et al.Mol.Cell Biol.24, 7902-7913. (2004)).4. Collaborating with Dr.Maita in Kyusyu Univ., we firstly reported the crystal structure of endonuclease domain of TRAS1 (Maita, N., et al.J.Biol.Chem.279, 41067-41076. (2004)).5.We established the retrotransposion assay system for 28SrDNA specific LINE, R1, which enable us to use several target specific LINE, telomere specific LINEs, SART1 and TRAS1,and rDNA specific R1,for future analysis (Anzai T., et al.Nucleic Acids Res.33, 1993-2002. (2005)).