Genetic factors responsible for the mobility of mPing in the rice genome

导致水稻基因组中 mPing 迁移的遗传因素

基本信息

批准号：
15380006
负责人：
OKUMOTO Yutaka
金额：
$ 9.09万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380006/
关键词：
Rice Transposon MITEs mechanism of transposition Mutation Rurm1 mPing MITE 転移

项目摘要

In a mutant line IM294 (slender glume mutant) which harbors Rurm1 (Rice Ubiquitine Related Modifier-1) allele inactivated by mPing insertion shows high excision frequency (ca.1%) of mPing from mutant Rurm1 allele (Rurm1^m). Expression levels of two ORFs of Ping were also high in IM294 comparing to the original variety Gimbozu. To clarify the relation between the function of Rurm1 and Ping activity under the same genetic background, the mutant allele of Rurm1 was introduced into Gimbozu background through recurrent backcrossing. Also, a pair of non-slender glume line (Rurm1^+/Rrum1^+) and slender glume line (Rurm1^m/Rurm1^m) derived from offspring of the non-slender glume plant (Rurm1^+/Rurm1^m) segregated among IM294 line were used. Comparing the Gimbozu and its isogenic line and sib lines, inactivation of Rurm1 with mPing insertion enhanced the expression levels of two ORFs of Ping. On the other hand, significant change in the expression level of Pong was not observed. These indicate that the inactivation of Rurm1 promotes the mPing excision by enhancing the expression of Ping's ORFs. Though, the inactivation of transposon is generally regulated by methylation, it is quite unlikely that RURM1 protein directly regulate the methylation of DNA. Effect of the inactivation of Rurm1 allele was also analyzed using 22k-micro array (Agilent Co.,). Inactivation of Rurm1 upregulate the expression of protein biosynthesis related genes in ribosome and nucleus. It also downregulated the expression of genes related to metabolism and physiological process. These indicate that the function of Rurm1 is related to transcription of vast number of genes related to the physiological process. Upregulation of protein synthesis related genes indicates the compensation to growth retardation of plants. Upregulation of genes related to DNA-repair process was also observed indicating the frequent DNA double strands break induced by mPing excision in IM294.

在含有Mping插入的RURM1（水稻与泛素相关的修饰符1）等位基因的突变线IM294（细长的胶胶突变体）中，MPing插入失活的等位基因显示出很高的切除频率（Ca.1％）的MPIS中的MPIS中的MPIT来自Mutant Rurm1等位基因（RURM1^m）。与原始品种gimbozu相比，IM294的两个ORF的表达水平也很高。为了阐明在相同的遗传背景下RURM1的功能与PING活性之间的关系，通过经常性的反向交叉将RURM1的突变等位基因引入了Gimbozu背景中。此外，使用了IM294系列中的一对非倾斜胶线（rurm1^+/rrum1^+）和细长的胶合线（rurm1^m/rurm1^m），这些后代（rurm1^+/rurm1^m）源自IM294系列中的非范围内glume植物（rurm1^+/rurm1^m）。比较gimbozu及其同源线和SIB线，将RURM1与MPing插入灭活增强了PING两个ORF的表达水平。另一方面，未观察到乒乓球水平的显着变化。这些表明RURM1的失活通过增强Ping ORF的表达来促进MPINC切除。但是，转座子的失活通常受甲基化调节，RURM1蛋白不可能直接调节DNA的甲基化。还使用22k-Micro阵列（Agilent Co.，）分析了RURM1等位基因失活的影响。 RURM1的失活上调核糖体和核中蛋白质生物合成相关基因的表达。它还下调了与代谢和生理过程有关的基因的表达。这些表明RURM1的功能与与生理过程有关的大量基因的转录有关。蛋白质合成相关基因的上调表明植物的生长迟钝的补偿。还观察到了与DNA修复过程相关的基因上调，这表明IM294中MPing Concision诱导的频繁DNA双链断裂。