Origin and activation mechanism of an active transposition of mPingin rice

mPingin水稻主动转座的起源和激活机制

• 批准号: 18380005
• 负责人: OKUMOTO Yutaka
• 金额: $ 8万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380005/
• 关键词: rice; transposon; transposable activity; mPing; QTL analysis; genus Oryza; ゲノム; MITE; 種分化; 転移因子

The distribution of miniature Ping (mPing) was studied in 50 wild Oryza accessions which encompassed 19 species representing nine genomes of genus Oryza. The mPing element was presented in all the accessions arid they were highly (more than 99%) similar each other. There are three major types of mPing elements; type 1 with 430bp originally found in Gimbozu (Osativa), type 2 with 430bp with 9bp base substitution al the position of 242-252, type 3 with 419bp with 11bp deletion at the position of 239-249. Type 2 and 3 are presented only in O.sativa and O.rugipogon, The wide presence of type 1 mping in all the other accession indicated mPing elements should be originated prior to the differentiation of the genus Oryza. Transposition and amplification of mPing and Ping are still underway in rice variety Gimbozu irrespective of its high copy number of mPing element (over 1000). In Gimbozu, more than 50 new transposition estimated to be occurred per plant per generation while no transposition activity of mPing was observed in Nippcnbare, To elucidate the genetic factors contributing to the transposition activity of mPing Gimbozu, F5 progeny lines of selfed F4 plants derived form the cross between Nipponbare and Gimbozu were used. The mPing transposition activity was evaluated from a number of new mPing insertions that were not presented in parental plants. Then, genetic map of Nipponbare x Gimbozu were constructed based on the insertion polymorphism of mPing (mPingSCAR marker) between two varieties. Results of a QTL analysis for the mPing activity revealed two major QTLs at chr 1 and chr4.

对稻属9个基因组的19个种的50份野生稻材料进行了微型Ping（mPing）的分布研究。mPing基因在所有材料中都有表达，且材料间的相似性在99%以上。mPing元件主要有三种类型：第一种为430 bp，最初发现于金保津（Osativa），第二种为430 bp，在242-252位有9 bp碱基替换，第三种为419 bp，在239-249位有11 bp缺失。第2和第3类mPing基因仅存在于栽培稻和野稻中，而第1类mPing基因在其他材料中广泛存在，表明mPing基因可能起源于稻属分化之前。mPing和Ping的转座和扩增在水稻品种Gimbozu中仍在进行中，尽管其mPing元件的高拷贝数（超过1000）。在Gimbozu中，估计每代每株植物发生超过50个新的转座，而在Nipponbare中没有观察到mPing的转座活性。为了阐明mPing Gimbozu转座活性的遗传因素，使用来自Nipponbare和Gimbozu之间杂交的自交F4植物的F5后代系。从亲本植物中不存在的许多新的mPing插入评价mPing转座活性。利用mPing（mPingSCAR标记）在两个品种间的插入多态性构建了日本晴×金宝津的遗传图谱。对mPing活性的QTL分析结果显示，在chr 1和chr 4处存在两个主效QTL。

项目成果

Rice Biology in the Genomics

基因组学中的水稻生物学

• 发表时间: 2008
• 作者: Nakazaki;T.;K. Naito;Y. Okumoto;and T. Tanisaka
• 通讯作者: and T. Tanisaka

Rurm1(Rice ubiquitin-related modifier-1)の機能喪失はmPingの転移を促す

Rurm1（水稻泛素相关修饰剂-1）功能丧失促进 mPing 转移

• 发表时间: 2006
• 作者: Yuki;Monden;田村佳奈子;門田有紀;奥本 裕;堀端章;内藤健;田村佳奈子
• 通讯作者: 田村佳奈子

mPingSCARマーカーはジャポニカイネ品種の分子マーカーとして極めて有用である

mPingSCAR 标记作为粳稻品种的分子标记非常有用

• 发表时间: 2007
• 作者: Nakazaki;T.;K.;Naito;Y.;Okumoto;T.;Tanisaka;池田裕樹;門田有希・内藤健・斉藤大樹・大木信彦・出田収・中崎鉄也・築山拓司・奥本裕・谷坂隆俊
• 通讯作者: 門田有希・内藤健・斉藤大樹・大木信彦・出田収・中崎鉄也・築山拓司・奥本裕・谷坂隆俊

Dramatic amplification of a rice transposable element during recent domestication

• DOI: 10.1073/pnas.0605421103
• 发表时间: 2006-11-21
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Naito, Ken;Cho, Eunyoung;Wessler, Susan R.
• 通讯作者: Wessler, Susan R.

Active transposon in rice, Rice Biology in the Genomics Era

水稻活性转座子，基因组时代的水稻生物学

• 期刊: Springer-Verlag Berlin Heidelbelg, H.Y.Hirano ed 38
• 作者: Nakazaki;T.;K.;Naito;Y.;Okumoto;T.;Tanisaka
• 通讯作者: Tanisaka