Origin and activation mechanism of an active transposition of mPingin rice

mPingin水稻主动转座的起源和激活机制

• 批准号: 18380005
• 负责人: OKUMOTO Yutaka
• 金额: $ 8万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380005/
• 关键词: rice; transposon; transposable activity; mPing; QTL analysis; genus Oryza; ゲノム; MITE; 種分化; 転移因子

The distribution of miniature Ping (mPing) was studied in 50 wild Oryza accessions which encompassed 19 species representing nine genomes of genus Oryza. The mPing element was presented in all the accessions arid they were highly (more than 99%) similar each other. There are three major types of mPing elements; type 1 with 430bp originally found in Gimbozu (Osativa), type 2 with 430bp with 9bp base substitution al the position of 242-252, type 3 with 419bp with 11bp deletion at the position of 239-249. Type 2 and 3 are presented only in O.sativa and O.rugipogon, The wide presence of type 1 mping in all the other accession indicated mPing elements should be originated prior to the differentiation of the genus Oryza. Transposition and amplification of mPing and Ping are still underway in rice variety Gimbozu irrespective of its high copy number of mPing element (over 1000). In Gimbozu, more than 50 new transposition estimated to be occurred per plant per generation while no transposition activity of mPing was observed in Nippcnbare, To elucidate the genetic factors contributing to the transposition activity of mPing Gimbozu, F5 progeny lines of selfed F4 plants derived form the cross between Nipponbare and Gimbozu were used. The mPing transposition activity was evaluated from a number of new mPing insertions that were not presented in parental plants. Then, genetic map of Nipponbare x Gimbozu were constructed based on the insertion polymorphism of mPing (mPingSCAR marker) between two varieties. Results of a QTL analysis for the mPing activity revealed two major QTLs at chr 1 and chr4.

在50个野生Oryza饰品中研究了微型PING（MPIN）的分布，其中包括19种代表Oryza属的基因组的物种。 MPIT元件是在干旱的所有配件中提出的，它们相似（超过99％）。 Mping元素有三种主要类型；最初在gimbozu（Osativa）中发现的430bp的类型1，带有430bp的2型242-252的位置为430bp，其位置为419bp，在239-249的位置为419bp且11bp删除。第2和3型仅在O.Sativa和O.Rugipogon中呈现，在所有其他登录中的1型MPing的广泛存在应起源于Oryza属的分化之前。在大米品种gimbozu中，MPIN和PING的换位和放大仍在进行中，无论其高拷贝数的MPing元素的高拷贝数（超过1000）。在Gimbozu中，估计有50多个新的换位是每一代植物发生的，而在Nippcnbare中未观察到MPIT的换位活性，以阐明遗传因素有助于Mping gimbozu的转置活性，F5 F4植物的F5后代线生长的F4植物派生的F5后代派生派生的交叉形成了Nipponbare和Gimbozu之间的交叉。从父母植物中未介绍的许多新的MPAD插入中评估了MPing转置活性。然后，基于两个品种之间的MPing（Mpingscar标记）的插入多态性构建Nipponbare X gimbozu的遗传图。 MPIT活性的QTL分析的结果表明，ChR 1和ChR4时有两个主要的QTL。

项目成果

Rice Biology in the Genomics

基因组学中的水稻生物学

• 发表时间: 2008
• 作者: Nakazaki;T.;K. Naito;Y. Okumoto;and T. Tanisaka
• 通讯作者: and T. Tanisaka

Rurm1(Rice ubiquitin-related modifier-1)の機能喪失はmPingの転移を促す

Rurm1（水稻泛素相关修饰剂-1）功能丧失促进 mPing 转移

• 发表时间: 2006
• 作者: Yuki;Monden;田村佳奈子;門田有紀;奥本 裕;堀端章;内藤健;田村佳奈子
• 通讯作者: 田村佳奈子

Dramatic amplification of a rice transposable element during recent domestication

• DOI: 10.1073/pnas.0605421103
• 发表时间: 2006-11-21
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Naito, Ken;Cho, Eunyoung;Wessler, Susan R.
• 通讯作者: Wessler, Susan R.

mPingSCARマーカーはジャポニカイネ品種の分子マーカーとして極めて有用である

mPingSCAR 标记作为粳稻品种的分子标记非常有用

• 发表时间: 2007
• 作者: Nakazaki;T.;K.;Naito;Y.;Okumoto;T.;Tanisaka;池田裕樹;門田有希・内藤健・斉藤大樹・大木信彦・出田収・中崎鉄也・築山拓司・奥本裕・谷坂隆俊
• 通讯作者: 門田有希・内藤健・斉藤大樹・大木信彦・出田収・中崎鉄也・築山拓司・奥本裕・谷坂隆俊

Transposon display of mutations on quantitative trait induced by the endogenous active transposon, mPing in rice (Oryza saliva)

水稻（Oryza saliva）中内源活性转座子 mPing 诱导的数量性状突变的转座子展示

• 发表时间: 2006
• 作者: Horibata;A.;K.;Matsui;E.;Inoue;T.;Yoshihiro;H.;Kawaji;M.;Nakagawa;Y.;Okumoto;T.;Nakazaki;T.;Tanisaka
• 通讯作者: Tanisaka