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Investigation of signal transduction pathways of jasmonic acid in higher plants

高等植物茉莉酸信号转导途径的研究

基本信息

批准号：
15380080
负责人：
YAMANE Hisakazu
金额：
$ 9.47万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In this project, in order to have basic information on jasmonic acid (JA) functions and its signal transduction pathways, we have performed extensive studies including promoter analysis of a jasmonic acid (JA)-responsive gene, functional identification of a JA biosynthetic enzyme gene, and detailed characterization of JA/elicitor-responsive transcription factors that play key roles in the regulation of defense responses in rice. The principal conclusions are as follows: (l) We identified a TGACG-containing sequence that is a cis element involved in the JA-responsiveness of OsOPR1 in suspension-cultured rice cells, suggesting that a basic leucine zipper (bZIP) transcription factor is involved in the JA-responsive expression of OsOPR1 transcription. (2) We provided evidence that an OPR family gene in rice chromosome 8, OsOPR7, encodes the enzyme involved in the JA biosynthesis. The expression of OsOPR7mRNA was induced and reached maximum levels within 0.5 h of mechanical wounding and dro … More ught stress, and the endogenous JA level started to increase in accordance with the increase in OsOPR7expression. (3) We indicated that the JA-responsive bHLH transcription factor gene RERJ1 isolated from suspension-cultured rice cells was expressed in response to exogenous JA in leaves, leaf sheaths, and roots of rice in a vegetative stage, and that RERJ1 expression in the leaves was up-regulated by exposure to wounding and drought stress. In our recent studies including microarray analysis using transgenic rice overexpressing sense-RERJ1 mRNA, it has been shown that RERJ1 is involved in regulation of pathogenesis-related protein genes expression. (4) We provided strong evidence for the involvement of OsWRKY53 and OsWRKY71 in regulating the expression of defense-related genes induced by chitin oligosaccharide and fungal cerebroside elicitors in rice. Although OsWRKY53 was suggested to be a transcriptional activator, OsWRKY71 showed features characteristic of a transcriptional repressor, indicating that the activation of defense-related genes by OsWRKY71 was probably indirect. Less

在本项目中，为了了解茉莉酸（jasmonic acid， JA）功能及其信号转导途径的基本信息，我们进行了广泛的研究，包括茉莉酸（jasmonic acid， JA）响应基因的启动子分析，茉莉酸生物合成酶基因的功能鉴定，以及在水稻防御反应调控中起关键作用的茉莉酸/激发子响应转录因子的详细表征。主要结论如下：(1)在悬浮培养的水稻细胞中，我们发现了一个含tgacg的序列，该序列是参与OsOPR1 ja响应性的顺式元件，这表明一个碱性亮氨酸拉链（bZIP）转录因子参与了OsOPR1转录ja响应性的表达。(2)我们提供的证据表明，水稻8号染色体上的一个OPR家族基因OsOPR7编码参与JA生物合成的酶。OsOPR7mRNA的表达受到诱导，在机械损伤后0.5 h内达到最高水平。更多的光胁迫，内源JA水平开始随着OsOPR7mRNA表达的增加而升高。(3)从悬浮培养的水稻细胞中分离到的响应JA的bHLH转录因子基因RERJ1在水稻营养期的叶片、叶鞘和根系中响应外源JA的表达，并且在叶片中受伤害和干旱胁迫后表达上调。在我们最近的研究中，包括使用过表达sense-RERJ1 mRNA的转基因水稻的微阵列分析，已经表明RERJ1参与调控发病相关蛋白基因的表达。(4)我们提供了强有力的证据，证明OsWRKY53和OsWRKY71参与调控几丁质寡糖和真菌脑苷激发子诱导的水稻防御相关基因的表达。虽然OsWRKY53被认为是一个转录激活因子，但OsWRKY71表现出转录抑制因子的特征，这表明OsWRKY71激活防御相关基因可能是间接的。少