Molecular diet analysis of larvae of important marine animals

重要海洋动物幼体的分子饮食分析

• 批准号: 15380137
• 负责人: ICHII Taro
• 金额: $ 5.18万
• 依托单位: Incorporated Administrative Agency Fisheries Research Agency
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380137/
• 关键词: spiny lobster; eel; squid; tuna; larvae; stomach content; genome typing

Since species identification of the host larvae is important to investigate the stomach content, we have developed molecular markers to determine species of spiny lobsters, cephalopods, lantern fishes and eels. Especially, identification of squid larvae significantly contributed to stock assessment and dynamics of squid and fishes utilizing squids. Phylogenetic analysis of tuna species was performed using novel nuclear DNA. Larvae of neon squid, eel, conger eel, scyllarid lobsters and palinurid lobsters were used for molecular diet analysis. In all larvae, fungi and host variant 18S rDNA were detected, and no candidate organism as prey was found in the squid and eels. On the other hand, 18S rDNA of cnidaria and urochordata were found in two (Panulirus longipes bispinosus and P.echinatus) of three palinurid species and one (Scyllarides sp.) of two scyllarid lobster species. Fish DNA was found in the Japanese spiny lobster (P.japonicus).

由于宿主幼虫的种类鉴定对胃内容物的调查很重要，我们已经开发了分子标记来确定刺龙虾，头足类，灯笼鱼和鳗鱼的种类。特别是，鱿鱼幼虫的识别显着有助于鱿鱼和利用鱿鱼的鱼类的资源评估和动态。利用新的核DNA进行金枪鱼物种的系统发育分析。以氖柔鱼、鳗鱼、海鳗、海螯虾和褐龙虾的幼体为材料，进行分子饲料分析。在所有的幼虫，真菌和宿主变异18S rDNA检测，没有发现候选生物作为猎物的鱿鱼和鳗鱼。另一方面，在3种棘尾类动物中的2种（长柄龙虾Panulirus longipes bispinosus和棘尾龙虾Panulirus echinatus）和1种（圆蟹Scyllarides sp.）两种海螯虾在日本刺龙虾（P. piny lobster）中发现了鱼类DNA。

项目成果

Eukaryotes from the hepatopancreas of palinurid lobster phyllosoma larvae.

来自青虾叶状体幼虫肝胰腺的真核生物。

• 发表时间: 2006
• 期刊: Proceedings of the International Workshop on Spiny Lpbster Seed production Technology (印刷中)
• 作者: Suzuki;N.
• 通讯作者: N.

PCR primers for fish G6PD gene intron and characterization of intron length variation in the albacore Thunnuns alalunga.

鱼类 G6PD 基因内含子的 PCR 引物以及长鳍金枪鱼 alalunga 内含子长度变异的表征。

• 发表时间: 2004
• 期刊: Molecular Ecology Notes 4
• 作者: Chow;S.
• 通讯作者: S.

Morphological description of a later stage phyllosoma larvae of Panulirus echinatus Smith, 1869 identified by DNA analysis.

1869 年通过 DNA 分析鉴定出的 Panulirus echinatus Smith 后期叶状体幼虫的形态学描述。

• 发表时间: 2006
• 期刊: Journal of Plankton Research (in press)
• 作者: Konishi;K.
• 通讯作者: K.

Genetic and morphological identification of larval and small juvenile tunas (Pisces : Scombridae) caught by a mid-water trawl in the western Pacific.

西太平洋中层拖网捕获的幼鱼和小型金枪鱼（双鱼座：鲭科）的遗传和形态学鉴定。

• 发表时间: 2003
• 期刊: Bulletin of Fisheries Research Agency 8
• 作者: Chow;S.
• 通讯作者: S.

Chow, S.et al.: "Genetic and morphological identification of larval and small juvenile tunas (Pisces : Scombridae) caught by a mid-water trawl in the western Pacific."Bulletin of Fisheries Research Agency. 8. 1-14 (2003)

Chow, S.等人：“西太平洋中层水域拖网捕获的幼体和小型金枪鱼（双鱼座：鲭科）的遗传和形态学鉴定。”渔业研究机构公报。