Molecular diet analysis of larvae of important marine animals

由于宿主幼虫的种类鉴定对胃内容物的调查很重要，我们已经开发了分子标记来确定刺龙虾，头足类，灯笼鱼和鳗鱼的种类。特别是，鱿鱼幼虫的识别显着有助于鱿鱼和利用鱿鱼的鱼类的资源评估和动态。利用新的核DNA进行金枪鱼物种的系统发育分析。以氖柔鱼、鳗鱼、海鳗、海螯虾和褐龙虾的幼体为材料，进行分子饲料分析。在所有的幼虫，真菌和宿主变异18S rDNA检测，没有发现候选生物作为猎物的鱿鱼和鳗鱼。另一方面，在3种棘尾类动物中的2种（长柄龙虾Panulirus longipes bispinosus和棘尾龙虾Panulirus echinatus）和1种（圆蟹Scyllarides sp.）两种海螯虾在日本刺龙虾（P. piny lobster）中发现了鱼类DNA。

Since species identification of the host larvae is important to investigate the stomach content, we have developed molecular markers to determine species of spiny lobsters, cephalopods, lantern fishes and eels. Especially, identification of squid larvae significantly contributed to stock assessment and dynamics of squid and fishes utilizing squids. Phylogenetic analysis of tuna species was performed using novel nuclear DNA. Larvae of neon squid, eel, conger eel, scyllarid lobsters and palinurid lobsters were used for molecular diet analysis. In all larvae, fungi and host variant 18S rDNA were detected, and no candidate organism as prey was found in the squid and eels. On the other hand, 18S rDNA of cnidaria and urochordata were found in two (Panulirus longipes bispinosus and P.echinatus) of three palinurid species and one (Scyllarides sp.) of two scyllarid lobster species. Fish DNA was found in the Japanese spiny lobster (P.japonicus).