Study on anti-allergic activity of DNA from immunobiotics and its application for biodefence foods.

最近的兴趣集中在肠道免疫对宿主防御的重要性上，但迄今为止，对潜在的机制知之甚少。Toll样受体（TLR）家族通过识别细菌病原体相关分子模式（PAMPs）在细菌防御中发挥重要作用。在这项研究中，食品的生理功能已经调查了免疫生物乳酸菌（LAB）通过TLR的免疫调节作用。在猪（预期作为人类模型）中进行的研究已通过免疫生物素LAB检查了肠道免疫调节。我们的研究现在已经证明了肠道中TLR介导的肠道免疫调节。在此基础上，我们还致力于建立一种免疫活性乳酸菌的分子免疫检测系统，并从免疫活性乳酸菌中寻找新的免疫刺激DNA序列。在体外和体内研究表明，免疫刺激DNA序列的免疫生物素的抗过敏活性。本项目的发现可能为进一步阐明免疫刺激DNA激活先天免疫反应的机制提供动力。此外，从乳酸菌中鉴定免疫调节因子，并通过细胞因子网络更好地了解肠道免疫调节，在食品免疫学的基础研究和开发“免疫生物食品”以预防过敏性疾病方面有希望。

Recent interest has focused on the importance of intestinal immunity for the host defense, but to date, not much has been known about the underlying mechanisms. Toll-like receptor (TLR) family plays an important role in the defense through recognizing bacterial pathogen associated molecular patterns (PAMPs). In this study, the physiological function of food products has investigated the immunoregulatory effects of immunobiotic lactic acid bacteria (LAB) via TLRs. Studies in swine, which is expected as a human model, have been examined intestinal immunoregulation by the immunobiotic LAB. Our research has now demonstrated regulation of intestinal immunity mediated by TLRs in the gut. On the basis of this study, efforts have also been made to develop a molecular immunoassay system for immuobiotic LAB and find novel immunostimulatory DNA sequences from imunobiotics. In vitro and in vivo study demonstrated an anti-allergic activity of immunostimulatory DNA sequences from imunobiotics. The findings in this project may provide impetus to further delineate the activation mechanism of the innate immune response with immunostimulatory DNA. In addition, identification of immunoregulatory factors from LAB, and better understanding of intestinal immune regulation through cytokine networks hold promise in basic research of food immunology and development of "immunobiotic foods" to prevent allergic disease.