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Development of a diagnostic system for definitive hosts of taeniid cestodes and an epizootiological survey for echinococcosis in urban area

绦虫终宿主诊断系统的建立及城市包虫病流行病学调查

基本信息

批准号：
15380205
负责人：
NONAKA Nariaki
金额：
$ 10.3万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380205/
关键词：
Echinococcosis Diagnosis Dog Fox PCR Coproantigen DNA Deworming 糞便排泄動物多包条虫終宿主テニア動物疫学虫卵

项目摘要

Echinococcus multilocularis is a zoonotic parasite endemic in Hokkaido. The definitive host of the parasite carnivore animal such as fox and dog, and the intermediate host is rodent. Humans get infection by accidentally ingestin; the parasite eggs. The prevalence in foxes in Hokkaido was about 40% in the last decade and that in pet dogs is 0.3-0.7% In response to a deteriorating situation, reporting system for canine echinococcosis has been enforced since 2004 in Japan. This study has aimed to improve a diagnostic system for the definitive hosts by incorporating specific DNA detection and t, investigate an urban life cycle of the parasite and risk for infection in Sapporo and its suburbs. In research for diagnosti, system, PCR with specific primers for E. multilocularis and PCR-RFLP for other taeniid cestodes were developed for the egg of taeniid cestodes that were found in fecal egg examination but morphologically indistinguishable among the family. Moreover DNA diagnosis in prepatent period in which eggs are not excreted in host feces was shown to be feasible by examining th, post-dewormed feces. In addition, high reliability of a developing coproantigen detection kit which can be incorporated i the diagnostic system was shown using the samples collected in Japan and other countries. During the evaluation, a cat excretin E. multilocularis eggs were found at first in Japan. In investigation on an urban life cycle, high prevalence was found i foxes inhabiting in Sapporo and Otaru. Foxes showed geographical preference for selecting their den sites. Therefore, potentia home range of infected foxes, thus a risk area for Echinococcus infection in the urban area could be estimated.

多房棘球绦虫是北海道的一种地方性人畜共患寄生虫。以狐狸、狗等食肉动物为终宿主，以啮齿动物为中间宿主。人类通过不小心摄入寄生虫卵而感染。北海道的狐狸在过去十年中的患病率约为40%，宠物犬的患病率为0.3-0.7%。为了应对日益恶化的情况，日本自2004年以来一直实施犬包虫病报告制度。本研究的目的是通过结合特异性DNA检测和t，调查寄生虫的城市生活周期和札幌及其郊区的感染风险，以改善最终宿主的诊断系统。在诊断体系的研究中，采用特异性引物进行PCR。对粪检中发现但形态上不能区分的带绦虫卵进行多房绦虫和其他带绦虫的PCR-RFLP分析。此外，通过检查驱虫后的粪便，证明了在卵未排出宿主粪便的开放前期进行DNA诊断是可行的。此外，使用在日本和其他国家收集的样品，显示了可并入诊断系统的正在开发的粪抗原检测试剂盒的高可靠性。在评价过程中，猫排泄E。多房卵最早在日本发现。在对城市生活周期的调查中，发现居住在札幌和小樽的狐狸的患病率很高。狐狸表现出地理偏好选择他们的巢穴网站。因此，可以估计出狐狸感染棘球蚴的可能活动范围，从而估计出城市地区棘球蚴感染的危险区。