Study of prenatal exposure to diesel exhaust on male reproductive and central nerve systems of mice

产前柴油机尾气暴露对雄性小鼠生殖和中枢神经系统影响的研究

• 批准号: 15390042
• 负责人: TAKEDA Ken
• 金额: $ 9.54万
• 依托单位: Tokyo University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390042/
• 关键词: diesel exhaust; in utero exposure; central nerve system; male reproductive system; accessory reproductive gland; testosterone; multi-nuclear giant cells; spontaneous motor activity; Xist遺伝子; 脳内伝達物質; 雄性生殖器; 受動的回避行動; 組織学的解析; 脳組織; Caspase 3; 血

The effects of in utero exposure to diesel exhaust (DE) on the mouse male reproductive system and central nerve system were examined. Various markers that appear in pups were assessed. Pregnant ICR mice inhaled DE at DEP concentrations of 0.3, 1.0, or 3.0 mg DEP/m3 or clean air, as the control, on days 2-16 days post coitum. On postnatal day (PND) 28, the weight of the testes and accessory glands, and testosterone concentration in serum were significantly higher in the DE-exposed male pups. Testosterone concentration correlated significantly (P<0.01) with the expression of steroidogenic enzyme mRNAs, weight of the testes and male reproductive accessory glands, and daily sperm production. A spontaneous motor activity was changed intentionally, and the change of the dopamine, noradrenalin and the serotonin systems was admitted in the brain.These findings indicate that early stage mouse embryo exposure to DE affects male genital system and the cranial nerve system after birth.

本文研究了宫内暴露柴油机尾气对雄性小鼠生殖系统和中枢神经系统的影响。评估了幼仔中出现的各种标志物。ICR孕鼠在妊娠后2-16天吸入DEP浓度为0.3、1.0和3.0 mg DEP/m3的DE或清洁空气作为对照。在出生后第28天（PND），睾丸和附腺的重量，血清中的睾酮浓度显着较高的DE暴露的雄性幼崽。睾酮浓度与睾丸激素生成酶mRNA的表达、睾丸和雄性生殖附腺的重量以及每日精子产量呈显著正相关（P<0.01）。结果表明，DE对小鼠胚胎早期的雄性生殖系统和出生后的脑神经系统均有影响。

项目成果

科学

科学

• 发表时间: 2012
• 作者: Sato;H.;Ishibashi;H.;Vetere;F.;Nakamura;H.;Saito;M;大木聖子
• 通讯作者: 大木聖子

化学と教育

化学与教育

• 发表时间: 2006
• 作者: Nozawa T;Tsuji A;Tamai I ほか;武田厚司
• 通讯作者: 武田厚司

Diesel exhaust affects immunological action in the placenta of mice

柴油机尾气影响小鼠胎盘的免疫作用

• 发表时间: 2005
• 期刊: Environ Toxicol 20
• 作者: Fujimoto A;Tsukue N;Watanabe M;Sugawara I;Yanagisawa R;Takano H;Yoshida S;Takeda;K
• 通讯作者: K

Effect of diesel exhaust on development of fetal reproductive function in ICR female mice.

柴油机尾气对ICR雌性小鼠胎儿生殖功能发育的影响。

• 发表时间: 2004
• 期刊: J Health Sci. 50
• 作者: Tsukue N;Yoshida S;Sugawara I;Takeda K
• 通讯作者: Takeda K

Ken Takeda: "Endocrine-disrupting activity of chemicals in diesel exhaust and diesel exhaust particles"Environmental Science. 11,1. 033-045 (2004)

Ken Takeda：“柴油机尾气和柴油机尾气颗粒中化学物质的内分泌干扰活性”环境科学。