Donor vascular endothelial regeneration and chimerism induced by recipient bone marrow transplantation

受体骨髓移植诱导供体血管内皮再生和嵌合

• 批准号: 15390486
• 负责人: SAITO Kazuhide
• 金额: $ 8.06万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390486/
• 关键词: microcirculation injury; ischemia-reperfusion injury; renal vascular endothelial cells; rat bone marrow transplantation; GFP positive cells; stem cell transplantation; 腎虚血再灌流障害; 血管内皮細胞; シクロスポリン; 骨髄幹細胞; 腎虚血灌流障害; 血管内皮細胞障害; 単離腎潅流実験系; 実時間型生体顕微鏡 /

1. From the clinicopathological observation of antibody-mediated rejection in living donor ABO-incompatible kidney transplantation and ischemia-reperfusion injury in deceased donor kidney transplantation, We had analyzed and evaluated the process of the microcirculation injury and microvascular endothelial-cell damage.2. We had established the GFP(+) to (-) rat bone marrow transplantation model and evaluated the role of The bone marrow derived endothelial progenitor cells in the regeneration of the injured microvascular Cells in renal ischemia-reperfusion injury.3. We have revealed that the bone marrow derived endothelial cells have migrated and recovered the injured endothelial cells in renal ischemia-reperfusion injury. It was approved not only by static immunohinstopathological-method but also by dynamic-direct observation by real-time phase contrast microscopy.4. Our result have approved for the first time that the injured renal microvascular endothelial cells were restored by Bone-marrow derived cells and reconstructed functional microvasculature.These result will be useful for the clinical renal preservation and regeneration therapy for antibody-mediated rejection in ABO-incompatible kidney transplantation and ischemia-reperfusion injury in deceased donor kidney transplantation.

1。从抗体介导的供体无兼容的肾脏移植和缺血 - 再灌注损伤的抗体介导的排斥反应的临床病理学观察中，我们已经分析并评估了微循环损伤和微血管内皮细胞损伤的过程。2。我们已经建立了GFP（+）至（ - ）大鼠骨髓移植模型，并评估了骨髓衍生的内皮祖细胞在肾脏局部再灌注损伤中受伤的微血管细胞再生中的作用。3。我们已经透露，骨髓衍生的内皮细胞已在肾脏缺血再灌注损伤中迁移并恢复了受伤的内皮细胞。它不仅是通过静态免疫抑制病理学批准的，而且还通过实时阶段对比度显微镜进行动态直接观察。4。我们的结果首次批准了受伤的肾脏微血管内皮细胞通过骨头衍生细胞恢复和重建的功能性微血管系统恢复。这些结果将有助于临床肾脏保存和再生疗法，用于抗体介导的抗体介导的抑制作用，在抗体的肾脏不合时宜的肾脏运输型疾病中，将是有用的。

项目成果

Local delivery of angiotensin receptor blocker into the kidney ameliorates progression of experimental glomerulonephritis.

将血管紧张素受体阻滞剂局部递送至肾脏可改善实验性肾小球肾炎的进展。

• 发表时间: 2006
• 期刊: Kidney International 70
• 作者: J.Mahmood;F.Khan;S.Okada;N.Kumagai;T.Morioka T.Oite.
• 通讯作者: T.Morioka T.Oite.

Establishment of immortalized human glomerular endothelial cell lines and their application

永生化人肾小球内皮细胞系的建立及其应用

• 发表时间: 2005
• 期刊: Nephron Exp Nephrol 99
• 作者: Yao J;Harada T
• 通讯作者: Harada T

Synergistic effects of PDGF-BB and cAMP-elevating agents on expression of connexin 43 in mesanegial cells.

PDGF-BB 和 cAMP 升高剂对系膜细胞中连接蛋白 43 表达的协同作用。

• 发表时间: 2006
• 期刊: American Journal of Physiology : Renal Physiology 290
• 作者: J.Yao;M.Kitamura;M.Takeda;T.Oite;et al.
• 通讯作者: et al.

諏訪通博: "実時間型共焦点レーザー走査生体顕微鏡を用いたEnhanced Green Fluorescent Protein(EGFP)陽性細胞の同定法"新潟医学会雑誌. 118・12(印刷中). (2004)

Michihiro Suwa：“使用实时共聚焦激光扫描生物显微镜识别增强型绿色荧光蛋白 (EGFP) 阳性细胞”，《新泻医学会杂志》118, 12（出版中）。

Suwa M, et al.: "Regeneration of renal microvasculature by bone marrow derived endothelial cells following ischemia-reperfusion injury(IRI)"Journal of American Society of Nephrology. 14. 60A (2003)

Suwa M 等人：“缺血再灌注损伤 (IRI) 后骨髓来源的内皮细胞对肾微脉管系统的再生”美国肾脏病学会杂志。