Development of pulpal blood circulation by the simultaneous measurement of blood flow and blood oxygenation

本研究的目的是建立一种同时测量人牙髓血流量和血氧含量的方法。研究结果如下：1.利用激光多普勒血流仪(LDF)对牙髓血流检测范围进行了研究。为此，对拔除的人上中切牙根管内的人工血液循环进行了测量。将检查的牙齿的根尖部分切断，并将根管从根尖末端扩大到2 mm的切迹，直到牙釉质结合处(CEJ)的水平。将肝素化和稀释的人外周血通过牙根尖孔泵入，监测血流信号。当根管扩大到距根尖3 mm或4 mm时，几乎没有信号。随着根管扩大术的进展，血流信号明显增强。结果表明，激光共聚焦荧光检测人牙组织中PBF的范围有限。(168周)2.进行了一项研究，以获得有关人类牙髓中血液氧合的测量信息。为此，通过充填不同氧合水平的稀释血液的拔除牙齿的透射光强度。透过绿光的光反映了血液中的血红蛋白的数量，而透过红光的光与牙齿中血液的含氧量有关。此外，以绿光和红光为光源的人体上中切牙的透射式光体积描记(TLP)显示出不同的特性。(250wds)结果表明，使用不同波长的光的TLP可以检测到人类牙齿牙髓血的数量和血氧水平。

The purpose of this study was to establish the simultaneous measurement of blood flow and blood oxygenation in the human pulp. Results obtained were as follows :1. A study was performed to obtain information on the detection area of pulpal blood flow (PBF) using Laser Doppler flowmetry (LDF). For this purpose, the artificial blood circulation in the root canals of extracted human upper central incisors were measured. The apical portions of examined teeth were severed and the root canals were enlarged from the apical end to the 2 mm incisal to the level of the cement-enamel junction (CEJ). Heparinized and diluted human peripheral blood was pumped through the apical foramen of the teeth, and the blood flow signal was monitored. Almost no signal was detected when the root canal was enlarged 3 or 4 mm apical to the CEJ. The blood flow signal significantly increased with the progress of root canal enlargement. The results indicated that the detection area of PBF in human teeth using LDF is limited. (168 wds)2. A study was performed to obtain information on the measurement of blood oxygenation in the human pulp. For this purpose, the intensity of transmitted-light through the extracted tooth filled with diluted blood with different oxygenation level. The transmitted-light through green light reflected the amount of blood haemoglobin, while that through red light was related with the oxygenation level of the blood in the tooth. In addion, transmitted-light photoplethysmography (TLP) through the human upper central incisors showed different characteristic between the green light and red light as the light source. (250 wds)Results indicated that TLP using different wavelengths of the light could detect the amount and the blood oxygenation level of the pulpal blood in the human teeth.