Central nervous systems of tongue movement relating upper airway patency

本研究的目的是阐明与上呼吸道通畅有关的舌运动中枢神经系统，特别是舌骨和舌骨运动神经元。本研究以Wistar大鼠为研究对象。1.膝舌骨和膝舌骨运动神经元的中枢分布(辣根过氧化物酶示踪法)观察到膝舌骨运动神经元的中心位置位于舌下核腹内侧亚核的外侧区，从吻端到OBEX的距离为0.7~1.2 mm。2.P物质和5-羟色胺在出生后Wistar大鼠舌下核腹内侧亚核和腹大腹侧亚核的分布(免疫组织化学分析)Wistar大鼠在胚胎第19天和出生后第0、4、7、14、21、28和70天(每龄各5只)。舌下核腹内侧亚核和腹大亚核内P物质和5-羟色胺免疫反应终末的密度在19岁时较低，到P7逐渐增加，之后逐渐下降。3.NK1、5HT1a和5HT1b受体的表达(原位杂交)生后未发现NK1、5HT1a和5HT1b受体在舌下核腹内侧亚核和腹大亚核的表达变化

The purpose of this study was to clarify the central nervous systems of tongue movement relating upper airway patency, especially genioglossal and geniohyoid motoneurons. Wistar rats were used in this study.1.Central distribution of genioglossal and geniohyoid motoneurons (Horseradish Peroxidase tracing study)The central location of geniglossal motoneurons was observed in the lateral region of the ventromedial subnucleus in the hypoglossal nucleus, extending from 0.7 to 1.2 mm rostral to the obex. The central location of the geniohyoid motoneurons were subsequently observed in the ventrolareral subnucleus of the hypoglossal nucleus.2.Distribution of substance P and serotonin in the postnatal rat ventromedial and ventrolareral subnucleus of the hypoglossal nucleus (Immunohistochemical analysis)Wistar rats at embryonic (E) day 19 and postnatal (P) days 0,4,7,14,21,28 and 70 (five rats at each age) were used. The density of both substance P- and serotonin- immunoreactive terminals in the ventromedial and ventrolareral subnucleus of the hypoglossal nucleus was low at E19, gradually increased until P7 and decreased thereafter.3.Expression of NK1,5HT1A and 5HT1B receptors (In situ hybridization)Postnatal change of expression of NK1,5HT1A and 5HT1B receptors was not recognized in the ventromedial and ventrolareral subnucleus of the hypoglossal nucleus