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Gene expression and neural circuit formation in the developing brain nuclei governing suckling and masticatory behavior.

发育中的脑核中控制哺乳和咀嚼行为的基因表达和神经回路形成。

基本信息

批准号：
15390631
负责人：
SHIRAKAWA Tetsuo
金额：
$ 9.34万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1) Expression of neuropeptide Y (NPY) mRNA in the developing brain nuclei controlling feeding behaviorNPY mRNA expression was examined by in situ hybridization in the developing brain nuclei controlling feeding behavior from day 2 to day 10 of postnatal age (PND2-10). NPY mRNA expression in the hypothalamic arcuate nucleus (ARC) was significantly higher in fasted group than control group at PND2, 7 and 10. NPY mRNA expression in the nucleus of the solitary tract was increased in fasted group compared to control group at PND2 but there was no difference at PND7 and 10. NPY mRNA expression in the ARC of fasted group was reduced by a s.c. injection of insulin (5U/kg) or leptin (2 mg/kg).2) Catechol O-methyltransferase (COMT) mRNA expression in neonatal hypothalamus and an implication of estrogen receptorsCOMT mRNA expression was observed in the hypothalamic suprachiasmatic nucleus (SCN) throughout its rostrocaudal extent at PND1 and 2, and the mRNA levels decreased gradually until PND16. COMT mRNA was predominantly localized to the ventral and medial parts of the SCN. Neonatal COMT mRNA expression in the hypothalamus was reduced by a s.c. injection of 17β-estradiol or bisphenol A, and these effects were blocked by co-administration of ICI182780, a selective estrogen receptor antagonist.3) Effects of soft food and tooth-loss on neurogenesis in the adult dentate gyrus (DG)Four-week-old mice were subjected to feed powder diet for 10 weeks with or without removal of molars. They received daily injection of bromodeoxyuridine (BrdU) at 14 weeks of age for 12 consecutive days. BrdU-positive DG cells in these mice showed larger reduction in number than in control at 5 weeks after the BrdU injection and the ratio of neurons to the BrdU-positive cells decreased in the molarless mice.

1)应用原位杂交技术，检测了大鼠生后2 - 10天（PND 2 -10）发育中控制摄食行为的脑核团神经肽Y（NPY）mRNA的表达。在PND 2、7、10，禁食组下丘脑弓状核（ARC）NPY mRNA表达显著高于对照组。与对照组相比，禁食组孤束核NPY mRNA表达在PND 2时增加，但在PND 7和10时无差异。s.c. 2）新生大鼠下丘脑儿茶酚O-甲基转移酶（COMT）mRNA的表达及雌激素受体的意义COMT mRNA在PND 1、2时在下丘脑视交叉上核（SCN）的吻尾侧均有表达，表达水平逐渐下降，直至PND 16。COMT mRNA主要定位于SCN的腹侧和内侧部分。新生儿下丘脑COMT mRNA的表达被s.c.注射17β-雌二醇或双酚A，并且这些作用被共同施用选择性雌激素受体拮抗剂ICI 182780阻断。3）软食物和牙齿缺失对成年齿状回（DG）中神经发生的影响将4周龄的小鼠在去除或不去除磨牙的情况下进行饲料粉末饮食10周。在14周龄时，他们每天接受溴脱氧尿苷（BrdU）注射，连续12天。在这些小鼠中的BrdU阳性DG细胞在注射BrdU后5周显示出比对照组更大的数量减少，并且在无磨牙小鼠中神经元与BrdU阳性细胞的比率降低。