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Establishment of a study model for the recovery of oral functions in patients suffering from somatosensory disfunction

体感障碍患者口腔功能恢复研究模型的建立

基本信息

批准号：
11557164
负责人：
SHIRAKAWA Tetsuo
金额：
$ 8.19万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11557164/
关键词：
Multi-electrode dish Circadian rhythm Suprachiasmatic nucleus Neural network Nucleus of the solitary tract Reticular formation Hypoglossal nucleus Trigeminal nucleus 分散培養静置器官培養神経幹細胞

项目摘要

The present study was carried out to establish a study model for the recovery of oral functions in patients suffering from somatosensory disfunction.We used dissociated cell culture of rat suprachiasmatic nucleus (SCN) neurons because the function of the SCN as a circadian pacemaker has been investigated enthusiastically in both in vivo and in vitro. By using a multi-electrode dish, we measured circadian firing rhythms in individual SCN neurons for more than 2 weeks continuously. Cross-correlation analysis of spontaneous action potentials revealed that a neuron pair was functionally connected by synapses when their circadian rhythms were synchronized. No correlation was found between the paired neurons whose circadian rhythms were not synchronized. Desynchronization of the firing rhythms and the disappearance of the cross-correlation after a chronic blockade of neural communication by tetrodotoxin demonstrate a causal relationship between synaptic transmission and rhythm synchrony.Feeding-associated activation of neurons in the medulla oblongata was examined in both developing and adult rats, using the c-fos gene-encoded protein (Fos) as a marker. Neonates were isolated from mothers for 12h on postnatal day 3,7 and 14, and either breast-fed intensively or further isolated for another 3h, and sacrificed. Adult rats were fasted for 24 hours and either given food and water before sacrifice or sacrificed without food intake. In the breast-fed pups, Fos-immunoreactive (FI) neurons were predominantly localized in the nucleus of the solitary tract (NST) and dorsal medullary reticular formation (dRF). Satiated adult rats with food showed similar patterns of Fos expression in both NST and dRF.In contrast, much fewer FI cells were observed in the NST and dRF in the fasted pups and adults. The distinct localization of Fos in fed pups and adults indicates functional significance of the NST and dRF in the feeding behavior.

本研究采用大鼠视交叉上核（suprachiasmatic nucleus，SCN）神经元分离培养的方法，建立了一种恢复躯体感觉功能障碍患者口腔功能的研究模型。通过使用多电极盘，我们连续两周以上测量了单个SCN神经元的昼夜放电节律。自发动作电位的互相关分析表明，当神经元对的昼夜节律同步时，神经元对在功能上通过突触连接。没有相关性的配对神经元的昼夜节律不同步。放电节律的去极化和河豚毒素慢性阻断神经通讯后的交叉相关性消失表明突触传递和节律synchrony. Feeding相关激活的延髓神经元之间的因果关系在发育和成年大鼠进行了检查，使用c-fos基因编码的蛋白质（Fos）作为标记。新生儿在出生后第3、7、14天与母亲隔离12 h，然后进行母乳喂养或进一步隔离3 h，然后处死。成年大鼠禁食24小时，在处死前给予食物和水，或在不进食的情况下处死。在母乳喂养的幼崽，Fos免疫反应（FI）神经元主要位于孤束核（NST）和背侧延髓网状结构（dRF）。饱食组大鼠NST和dRF中Fos表达模式相似，而禁食组和成年组大鼠NST和dRF中Fos表达明显减少。Fos在摄食后的幼仔和成体中的明显定位表明NST和dRF在摄食行为中的功能意义。