Effects of LPS from periodontal lesion on activation of circulatory and immune cells.

牙周病变中的脂多糖对循环和免疫细胞活化的影响。

• 批准号: 15390642
• 负责人: NITTA Hiroshi
• 金额: $ 9.47万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390642/
• 关键词: LPS; Periodontitis; IL-6; CRP; エンドトキシン; LBP

1. Changes in levels of LPS and various inflammatory markers in blood by intervention of periodontal treatment.Seventeen chronic periodontitis non-smoker patients without any systemic disease were subjected for the study. Levels of plasma LPS, serum IL-6, CRP and LBP were determined before, immediately after and one month after the full mouth mechanical debridement.The results as follows :(1) LPS was detected in the plasma from 3 out of 17 patients.(2) The mean serum IL-6 level was elevated immediately after treatment, and reduced to the baseline level one month after treatment. The mean serum LPB level was reduced immediately after treatment, and returned to the baseline level one month after treatment. The mean CRP level was not significantly different during the study. Serum CRP levels of 5 patients were reduced after one month treatment.These results suggested that quantity of LPS come into the circulation from periodontal lesion was very small or LPS was immediately inactivated by LBP etc. However serum IL-6 level was elevated and LBP level was reduced immediately after treatment. Therefore bacteria or LPS from periodontal lesion affect systemically.2. Response of whole blood to low dose LPS.Blood samples obtained from seven healthy individuals were pretreated with or without 5pg/ml Aa LPS, followed by further stimulation with 1ng/ml Aa LPS. The concentrations of IL-1〓, IL-6, IL-10 and TNF-〓 in the culture supernatants were then determined.Pretreatment with 5pg/ml Aa LPS significantly enhanced the production of IL-1〓 and IL-6 from whole blood when further induced by 1ng/ml LPS.These results suggested that a low dose of bloodstream LPS found in periodontitis patients appears to be sufficient to have priming effect and may be capable of affecting the systemic responses of immune and inflammatory cells.

1.牙周治疗干预后血中脂多糖和各种炎症标志物水平的变化17例无全身性疾病的慢性牙周炎患者接受了这项研究。分别于术前、术后即刻及术后1个月测定血浆LPS、血清IL-6、CRP和LBP水平，结果：（1）17例患者中3例检测到LPS; (2)治疗后即刻血清IL-6水平升高，治疗后1个月降至基线水平。血清LPB水平治疗后即刻下降，治疗后1个月恢复至基线水平。研究期间平均CRP水平无显著差异。5例患者治疗1个月后血清CRP水平下降，提示牙周病损进入循环的LPS量很少或LPS被LBP等立即灭活，而治疗后血清IL-6水平升高，LBP水平下降。因此，牙周病损的细菌或脂多糖对牙周病的影响是系统性的.全血对低剂量LPS的反应：取7名健康人的血样，用5 pg/ml Aa LPS或不加LPS预处理，然后用1 ng/ml Aa LPS进一步刺激。IL-1 β、IL-6、用5 μ g/ml Aa LPS预处理可显著提高全血中IL-10和IL-6的产生，而用1 ng/ml Aa LPS进一步诱导则可显著提高全血中IL-10和IL-6的产生。这些结果表明，在牙周炎患者中发现的低剂量的血流LPS似乎足以具有引发效应，并且可能能够影响牙周炎患者的牙周组织。免疫和炎症细胞的全身反应。

项目成果

A novel mutation of the cathepsin C gene in a thai family with Papillon-Lefevre syndrome.

泰国 Papillon-Lefevre 综合征家族中组织蛋白酶 C 基因的新突变。

• 发表时间: 2005
• 期刊: J Periodontol 76・3
• 作者: Suzuki Y;Nitta H;et al.;Nitta H
• 通讯作者: Nitta H

Effect of low dose Actinobacillus actinomycetemcomitans LPS pre-treatment on cytokine production by human whole blood.

低剂量Actinobacillus actinomycetemcomitans LPS预处理对人全血细胞因子产生的影响。

• 发表时间: 2004
• 期刊: J Periodontal Res 39・2
• 作者: Koshy G;Nitta H;et al.;Nakamura T
• 通讯作者: Nakamura T

Transforming growth factor-β stimulates interleukin-11 production by human periodontal ligament and gingival fibroblasts

• DOI: 10.1111/j.1600-051x.2006.00898.x
• 发表时间: 2006-03-01
• 期刊: JOURNAL OF CLINICAL PERIODONTOLOGY
• 影响因子: 6.7
• 作者: Yashiro, R;Nagasawa, T;Ishikawa, I
• 通讯作者: Ishikawa, I

A tumor necrosis factor-α antagonist inhibits inflammatory bone resorption induced by Porphyromonas gingivalis infection in mice

• 发表时间: 2004
• 作者: 鈴木 賀文

A novel mutation of the cathepsin C gene in a Thai family with Papillon-Lefevre

泰国 Papillon-Lefevre 家系组织蛋白酶 C 基因的新突变

• 发表时间: 2005
• 期刊: J Periodontol 76・3
• 作者: H.Nitta;Wara-Aswapati N;et al.
• 通讯作者: et al.