Effects of LPS from periodontal lesion on activation of circulatory and immune cells.

1. 牙周治疗干预对血液中脂多糖和各种炎症标志物水平的影响。17例慢性牙周炎患者，不吸烟，无全身性疾病。测定全口机械清创前、术后即刻及术后1个月血浆LPS、血清IL-6、CRP及LBP水平。结果表明：(1)17例患者中有3例血浆中检测到LPS。(2)治疗后血清平均IL-6水平立即升高，治疗后1个月降至基线水平。平均血清LPB水平在治疗后立即降低，并在治疗后一个月恢复到基线水平。平均CRP水平在研究期间无显著差异。5例患者治疗1个月后血清CRP水平降低。这些结果表明，从牙周病变进入循环的LPS数量非常少或LPS被LBP立即灭活等。治疗后血清IL-6水平升高，LBP水平降低。因此，来自牙周病变的细菌或脂多糖具有全身性影响。全血对低剂量LPS的反应。7名健康个体的血液样本分别用或不加5pg/ml Aa LPS预处理，然后用1ng/ml Aa LPS进一步刺激。然后测定培养上清液中IL-1〓、IL-6、IL-10和TNF-〓的浓度。5pg/ml Aa LPS预处理后，再用1ng/ml LPS诱导，全血IL-1〓和IL-6的生成显著增加。这些结果表明，在牙周炎患者中发现的低剂量血液LPS似乎足以产生启动效应，并且可能能够影响免疫和炎症细胞的全身反应。

1. Changes in levels of LPS and various inflammatory markers in blood by intervention of periodontal treatment.Seventeen chronic periodontitis non-smoker patients without any systemic disease were subjected for the study. Levels of plasma LPS, serum IL-6, CRP and LBP were determined before, immediately after and one month after the full mouth mechanical debridement.The results as follows :(1) LPS was detected in the plasma from 3 out of 17 patients.(2) The mean serum IL-6 level was elevated immediately after treatment, and reduced to the baseline level one month after treatment. The mean serum LPB level was reduced immediately after treatment, and returned to the baseline level one month after treatment. The mean CRP level was not significantly different during the study. Serum CRP levels of 5 patients were reduced after one month treatment.These results suggested that quantity of LPS come into the circulation from periodontal lesion was very small or LPS was immediately inactivated by LBP etc. However serum IL-6 level was elevated and LBP level was reduced immediately after treatment. Therefore bacteria or LPS from periodontal lesion affect systemically.2. Response of whole blood to low dose LPS.Blood samples obtained from seven healthy individuals were pretreated with or without 5pg/ml Aa LPS, followed by further stimulation with 1ng/ml Aa LPS. The concentrations of IL-1〓, IL-6, IL-10 and TNF-〓 in the culture supernatants were then determined.Pretreatment with 5pg/ml Aa LPS significantly enhanced the production of IL-1〓 and IL-6 from whole blood when further induced by 1ng/ml LPS.These results suggested that a low dose of bloodstream LPS found in periodontitis patients appears to be sufficient to have priming effect and may be capable of affecting the systemic responses of immune and inflammatory cells.