A study of genetic risk factors for susccptibility to pcriodontal discascs by molccular biology

牙周盘易感性遗传危险因素的分子生物学研究

• 批准号: 12672028
• 负责人: NITTA Hiroshi
• 金额: $ 2.11万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672028/
• 关键词: periodontal disease; genetics; risk factors; macrophages; 歯周疾; 遺伝子型

The objectives of this study were to clarify the association of genotypes of CD14, TNF-α, and IL-1. And condition and types of pcriodontilis. And function of the macrophagcs.Fifty-five patients with adult pcriodonlitis (AP), 35 patients with early-onset periodontilis (EOP) and 40 healthy controls were participated in this study. Genotypes of CD 14, TNF-α, IL-1 were determined using PCR-RFLP method.The results as follows:1. The prevalence of the IL-1A^<-889> allele 2 genotype was greater in the patients than in controls. The difference between the EOF and the control groups was significant.2. The prevalence of the composite genotype comprising allele 2 of IL-1A^<-889> plus allclc 2 of IL IL-1B^<+3953> was greater in the patient group. The difference between the EOF and control groups was significant.3. No differences were observed between the patient and control groups, or types of periodontitis in distribution of genotypes of CD14, TNF-α.4. No association was observed between the genotypes and function of macrophagcs (production of TNF-α, IL-1α, IL-1β, soluble CD14 from peripheral-blood mononuclear cells stimulated with LPS. And expression intensity of CD 14 on macrophages analyzed using flow cytomelry).These results suggest that IL-1 genotype is a useful risk marker for periodonlal diseases including earlyonset periodontitis in Japanese population.

本研究的目的是阐明CD 14、TNF-α和IL-1基因型之间的相关性。以及牙周炎的情况和类型。对55例成人牙周炎（AP）、35例早发性牙周炎（EOP）和40例健康对照者进行了研究。采用PCR-RFLP方法检测CD 14、TNF-α、IL-1基因型。患者中IL-1A^<-889>等位基因2基因型的患病率高于对照组。实验组与对照组比较差异有显著性.在患者组中，包括IL-1A^的等位基因2 + <-889>IL-1B^&lt;+3953&gt;的allclc 2的复合基因型的患病率更高。实验组与对照组比较，差异有统计学意义. CD 14、TNF-α基因型分布在牙周炎患者组与对照组之间及牙周炎类型之间均无差异。基因型与巨噬细胞功能（TNF-α、IL-1α、IL-1β、可溶性CD 14）无相关性。这些结果表明IL-1基因型是日本人群牙周疾病包括早发性牙周炎的一个有用的危险标志。

项目成果

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Nitla H., H. Kato, M. Umeda, S. Kuwata, I. Ishikawa: "Papillon-Lefevre syndrome : Analysis of HLA antigens"Oral Dis.. 6. 278-281 (2000)

Nitla H.、H. Kato、M. Umeda、S. Kuwata、I. Ishikawa：“Papillon-Lefevre 综合征：HLA 抗原分析”Oral Dis.. 6. 278-281 (2000)

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