Long-term Culture of Multicellular Spheroids Applicable to an Alternative to Animal Experiments.

多细胞球体的长期培养适用于动物实验的替代。

• 批准号: 16300165
• 负责人: OTSUKA Hidenori
• 金额: $ 9.15万
• 依托单位: Tokyo University of Science (2006)National Institute for Materials Science (2004-2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16300165/
• 关键词: Biointerface; Microfabrication; Patterning; Spheroid; Cell Array; Hepatocyte; Photolithography; 軟骨; パターニング; 微小組織エレメント

We report here for the first time a simple and effective way to form a two-dimensional micro-array of ten thousand (100x100) hepatocyte hetero-spheroids. The hepatocyte hetero-spheroids were underlain with endothelial cells in an area of 20x20.mm, by using micro-fabricated glass substrates, coated with poly (ethylene glycol) (PEG) brushes. Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver specific function, allowing us to obtain hepatocyte spheroids with a diameter of 100-μm, functioning as a miniaturized liver to secret albumin for at least three weeks. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors (TBB and CBB), detecting a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response. This system can be used as an alternative to animal experiments in a variety of situations in which the reliance on animals would prove impractical. Furthermore, the isolated spheroids from the array may have potential utility in constructing a tissue engineered liver by seeding spheroids into three-dimensional scaffolds.

我们首次报道了一种简单而有效的方法来形成一万(100×100)个肝细胞异质球体的二维微阵列。采用自制的玻璃基板，聚乙二醇刷涂布，在20×20 mm的面积内，将肝细胞异种球体与内皮细胞包被。肝细胞与内皮细胞共培养是稳定肝细胞活力和肝特异性功能的关键，使我们能够获得直径为100-μm的肝细胞球体，作为分泌白蛋白至少三周的微型肝脏。这里构建的椭球阵列作为基于组织和细胞的生物传感器(TBB和CBB)的平台非常有用，通过细胞生理反应检测各种临床、药理和毒理活性化合物。在依赖动物被证明是不切实际的各种情况下，这个系统可以作为动物实验的替代方案。此外，通过将球体种植到三维支架中，从阵列中分离出的球体可能在构建组织工程肝脏方面具有潜在的用途。

项目成果

Hydroxyapatite-coated tendon chitosan tubes with adsorbed laminin peptides facilitate nerve regeneration in vivo

• DOI: 10.1016/j.brainres.2003.09.003
• 发表时间: 2003-12-12
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Itoh, S;Yamaguchi, I;Tanaka, J
• 通讯作者: Tanaka, J

ナノマテリアル・ハンドブック

纳米材料手册

• 发表时间: 2005
• 作者: 英謙二;白井汪芳
• 通讯作者: 白井汪芳

有機トランジスタ材料の評価と応用

有机晶体管材料的评价及应用

• 发表时间: 2005
• 作者: 岩佐 義宏(分担);青木優 等;木口学;岩佐 義宏(分担)
• 通讯作者: 岩佐 義宏(分担)

Muscle-specific protein MDP77 specifically promotes motor nerve regeneration in rats

• DOI: 10.1016/j.neulet.2004.02.058
• 发表时间: 2004-04-29
• 期刊: NEUROSCIENCE LETTERS
• 影响因子: 2.5
• 作者: Itoh, S;Uyeda, A;Taguchi, T
• 通讯作者: Taguchi, T

Physicochemical properties of gelatin gels prepared using citric acid derivative

• DOI: 10.1016/j.msec.2004.08.039
• 发表时间: 2004-12
• 期刊: Materials Science and Engineering: C
• 作者: H. Saito;T. Taguchi;Hisatoshi Kobayashi;K. Kataoka;J. Tanaka;S. Murabayashi;Y. Mitamura