Basic and Clinical Studies on Natural Antioxidant Astaxanthin on the Inhibition of Diabetic Complication

天然抗氧化剂虾青素抑制糖尿病并发症的基础与临床研究

• 批准号: 16300240
• 负责人: NGUYEN Van Chuyen
• 金额: $ 6.4万
• 依托单位: Japan Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16300240/
• 关键词: Diabetes; Astaxanthin; Tocopherol; Tocotrienol; Ascorbic acid; Flavengenol; Cataract; Antioxidant; 血糖値; 過酸化脂質; セサミン; メイラード反応

In this study, Astaxanthin, a kind of Carotenoid, was used in combination with other antioxidants such as Tocopherol, Tocotrienol, Ascorbic acid or Flavengenol (a mixture of polyphenols from pine) in diabetic rats, to confirm the effectiveness of these combinations against oxidative stress and diabetic complications.The experiments were carried out on STZ diabetic rats, then, the following results were confirmed : In the diabetic rats fed with Astaxanthin in combination with Tocopherol or Tocotrienol the strong inhibition of oxidative stress was observed. The 8-OHdG biomarker in urine of diabetic rats was also lower than that of the control. However, in the diabetic rats fed with Astaxanthin in combination with high dose of Ascorbic acid, the lipid peroxide was higher than that of the control. From this result, it can be concluded that high dose of Ascorbic acid accelerated the oxidation in vivo. Besides, by the combination with Astaxanthin, the comsumption of Tocopherol was reduced, t … More hen the concentration of Tocopherol in serum and internal organs were increased.Moreover, water soluble Flavengenol was used in the combination with Astaxanthin for the inhibition of diabetic complications. Then, the results showed that with the combination with Flavengenol, the lipid peroxide in liver, kidney and serum were lower than that of the control. The degree of cataract was also lower than that of the control. The 8-OHdG biomarker in urine was also lower than that of the control. From these results, Astaxanthin by the combination with Flavangenol, the inhibition of lipid peroxide in diabetic rats was significant.From these above results, Astaxanthin in the combination with Tocopherol, Tocotrienol or Flavengenol was effective for the inhibition of diabetic complications. Diabetes is increasing in developed and developing countries. For the inhibition of diabetic complications, it is hoped that the results in this study will be reference for the prevention method of diabetic complications. The clinical trials in human will be carried out in the coming year, in the hospital.. Less

本研究将类胡萝卜素--虾青素与其它抗氧化剂如生育酚、生育三烯酚、抗坏血酸或黄酮酚联合使用（来自松树的多酚的混合物）在糖尿病大鼠中的作用，以证实这些组合对抗氧化应激和糖尿病并发症的有效性。在用虾青素与生育酚或生育三烯酚组合喂养的糖尿病大鼠中，观察到氧化应激的强烈抑制。糖尿病大鼠尿中8-OHdG生物标志物也低于对照组。但在高剂量抗坏血酸与虾青素联合喂养的糖尿病大鼠中，脂质过氧化物高于对照组。从这个结果可以得出结论，高剂量的抗坏血酸加速了体内的氧化。此外，通过与虾青素的结合，降低了生育酚的消耗， ...更多信息 结果表明，水溶性黄酮酚能显著提高糖尿病大鼠血清和内脏中维生素E的含量，并与虾青素联合应用，抑制糖尿病并发症的发生。结果表明，与黄酮醇合用后，肝、肾和血清中的脂质过氧化物含量均低于对照组。白内障程度也低于对照组。尿中8-OHdG生物标志物也低于对照组。从以上结果可以看出，虾青素与黄烷醇联合应用对糖尿病大鼠脂质过氧化有显著的抑制作用，虾青素与生育酚、生育三烯酚或黄烷醇联合应用对糖尿病并发症的抑制作用明显。糖尿病在发达国家和发展中国家都在增加。为糖尿病并发症的防治提供参考。人体临床试验将于明年在医院进行。少