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Comparative genomics of gene expression mechanisms of parasitic protozoa using full-length cDNA

使用全长 cDNA 比较寄生原生动物基因表达机制的基因组学

基本信息

批准号：
16310132
负责人：
WATANABE Junichi
金额：
$ 9.98万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Parasitic protozoa are the simplest eukaryotes of which genomes have been sequenced because of their medical importance. As the attempt to study gene expression mechanism of eukaryotes, we have produced full-length cDNA libraries using the oligo-capping method which we have originated from various parasites including tertian malaria parasite, Plasmodium vivax, murine malaria parasite, P. berghei, Toxoplasma gondii, Cryptosporidium parvum, Theileria parva, T. orientalis, and Eimeria tenella, and determined 5'-end-one-pass sequences from the random clones. These sequences have been mapped onto the respective genome sequences and presented in the genome browsers. For comparative genomic analysis, a full-length cDNA library has been produced from flatworm, Echinococcus multilocularis. The results have been compiled as a database, Full-Malaria and published in internet at http : //fullmal.ims.u-tokyo.ac.jp. Furthermore, comparative genomics database, Comparasite, (http : //comparasite.hgc.ip) has been produced. These provide basic information for comparative genome analysis to elucidate the exact mechanisms of gene expression. In addition, using the original method to screen novel malaria DNA vaccine candidates, promising murine malaria vaccine candidate clones have been selected. This method should be applicable to other eukaryotic pathogens.

寄生原生动物是最简单的真核生物，由于其医学重要性，其基因组已被测序。作为研究真核生物基因表达机制的尝试，我们利用寡核苷酸加帽法构建了间日疟原虫、鼠疟原虫、伯氏疟原虫、弓形虫、隐孢子虫、小泰勒虫、鼠疟原虫、orientalis和柔嫩艾美耳球虫（Eimeria tenella），并从随机克隆中确定了5 '端一次通过序列。这些序列已被映射到各自的基因组序列，并在基因组浏览器中呈现。为了进行比较基因组分析，已经从扁虫多房棘球绦虫中产生了全长cDNA文库。研究结果已汇编成一个数据库，名为“全面疟疾”，并在互联网上公布，网址为http：//fullmal.ims.u-tokyo.ac.jp。此外，还建立了比较基因组学数据库Comparasite（http：//comparasite.hgc.ip）。这些为比较基因组分析提供了基本信息，以阐明基因表达的确切机制。此外，使用原始方法筛选新的疟疾DNA疫苗候选物，已经选择了有希望的小鼠疟疾疫苗候选物克隆。该方法也适用于其他真核生物病原体的检测。