Studies on development, structure and function of pituitary gland in amphibians
两栖类垂体发育、结构与功能的研究
基本信息
- 批准号:16370031
- 负责人:
- 金额:$ 8.45万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2004
- 资助国家:日本
- 起止时间:2004 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1.Ontogenic study of pituitary glandAs a tool for tracing the cells that migrate with pituitary primordium but remain in the foregut region, transgenic toads (Xenopus laevis) that express GFP were prepared.2.Central action of prolactinAn adenohypophyseal hormone prolactin is involved in eliciting courtship behavior in the newt, genus Cynops, acting centrally. As a step to elucidate the mechanism of the prolactin action, distribution of prolactin receptors in the newt (Cynops pyrrhogaster) brain was studied by immunohistochemistry and in situ hybridization. Immunohistochemically detectable prolactin receptors as well as prolactin receptor mRNAs were observed in several region of hypothalamic nuclei, and most notably in the choroid plexus. Involvement of choroid plexus in transporting prolactin molecules into the brain was suggested.3.Regulation of pituitary function by hypothalamic neuropeptidesNewly developed radioimmunoassay for amphibian TSH made it possible to detect TSH-releasing factors in the amphibian brain. As a result, CRF was revealed to be the most potent substance to stimulate the release of TSH from the bullfrog pituitary. Using CRF antagonists and agonists, CRF receptor type 2 was demonstrated to mediate the action of CRF to release TSH.
1.垂体的个体发育研究作为追踪随垂体原基迁移但保留在前肠区域的细胞的工具,制备了表达GFP的转基因蟾蜍(Xenopus laevis)。2.催乳素的中枢作用腺垂体激素催乳素在蝾螈属蝾螈的求偶行为中起中枢作用。作为阐明催乳素作用机制的一个步骤,通过免疫组织化学和原位杂交研究了蝾螈(Cynopspyrrhogaster)大脑中催乳素受体的分布。在下丘脑核的几个区域,尤其是在脉络丛中观察到免疫组织化学可检测的催乳素受体以及催乳素受体 mRNA。认为脉络丛参与了催乳素分子向大脑的转运。3.下丘脑神经肽对垂体功能的调节新开发的两栖动物TSH放射免疫分析方法使检测两栖动物脑内TSH释放因子成为可能。结果表明,CRF 是刺激牛蛙垂体释放 TSH 的最有效物质。使用 CRF 拮抗剂和激动剂,2 型 CRF 受体被证明可以介导 CRF 释放 TSH 的作用。
项目成果
期刊论文数量(61)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Expression of erythropoietin receptor-like molecule in Xenopus laevis and erythrocytopenia upon administration of its recombinant soluble form
- DOI:10.1093/jb/mvi113
- 发表时间:2005-08-01
- 期刊:
- 影响因子:2.7
- 作者:Aizawa, Y;Nogawa, N;Kato, T
- 通讯作者:Kato, T
In vitro effect of bisphenol A on the thyroid hormone receptor gene expression in the Xenopus tail and testis.
双酚A对非洲爪蟾尾部和睾丸甲状腺激素受体基因表达的体外影响。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:S.Iwamuro;N.Aikawa;M.Yamada;S.Kikuyama
- 通讯作者:S.Kikuyama
Molecular cloning of bullfrog corticotropin-releasing factor (CRF) : effect of homologous CRF on the release of TSH from pituitary cells in vitro.
牛蛙促肾上腺皮质激素释放因子(CRF)的分子克隆:同源CRF对体外垂体细胞释放TSH的影响。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:R.Urakawa;H.Toda;K.Haibara;Y.Ito et al.
- 通讯作者:Y.Ito et al.
Processing of multiple forms of preprosodefrin in the abdominal gland of the red-bellied newt Cynops pyrrhogaster : regional and individual differences in preprosodefrin.
红腹蝾螈腹腺中多种形式的前原索德林的加工:前原索德林的区域和个体差异。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:K.Iida;Y.Tang;T.Mukai;Y.Nishimori;T.Iwata et al.
- 通讯作者:T.Iwata et al.
Monoaminergic neuronal development is not affected in PACAP-gene-deficient mice
- DOI:10.1016/j.regpep.2004.08.034
- 发表时间:2005-03-15
- 期刊:
- 影响因子:0
- 作者:Ogawa, T;Nakamachi, T;Shioda, SJ
- 通讯作者:Shioda, SJ
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KIKUYAMA Sakae其他文献
KIKUYAMA Sakae的其他文献
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{{ truncateString('KIKUYAMA Sakae', 18)}}的其他基金
Development of the pituitary research in terms of regulation of its function in the amphibian as a model
以两栖动物为模型,垂体功能调节研究的进展
- 批准号:
21570071 - 财政年份:2009
- 资助金额:
$ 8.45万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Central action of proteinacious hormones
蛋白质激素的中枢作用
- 批准号:
18570063 - 财政年份:2006
- 资助金额:
$ 8.45万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Regulation of pituitary function by pituitary hormones
垂体激素对垂体功能的调节
- 批准号:
07454226 - 财政年份:1995
- 资助金额:
$ 8.45万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Experimental analysiss of morphogenesis of hypothalamohypophyseal system
下丘脑垂体系统形态发生的实验分析
- 批准号:
05454024 - 财政年份:1993
- 资助金额:
$ 8.45万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Endocrinological analysis of amphibian life cycle
两栖动物生命周期的内分泌分析
- 批准号:
01440005 - 财政年份:1989
- 资助金额:
$ 8.45万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
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