Structure-function relationships of a yeast putative stretch-activated calcium channel

酵母推定拉伸激活钙通道的结构-功能关系

• 批准号: 16370072
• 负责人: IIDA Hidetoshi
• 金额: $ 9.86万
• 依托单位: Tokyo Gakugei University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16370072/
• 关键词: Stretch-activated calcium channel; Calcium channel; Signal transduction; Saccharomyces cerevisiae; Arabidopsis thaliana; Calcium; Mid1; Cch1; Mca1; 機械刺激; イネ; Spa2; OsTpc1; 電位作動型カルシウムチャネル; 伸展活性化Ca^<2+>チャネル; 機械受容チャネル; Ca^<2+>チャネル; 電立依存性Ca^<

1) The amino acid residue Phe^<356> of the putative stretch-activated Ca^<2+> channel Mid1 was determined to be important for cell viability in yeast. Systematic substitution of various amino acids for Phe^<355> revealed that hydrophobicity of this residue is critical.2) The Mid1 protein was found to be localized to the plasma membrane and endoplasmic reticulum membrane. Mid1 forms a oligomer via disulfide bonding.3) A multicopy suppressor of the mid1 mutation was isolated and characterized. This multicopy suppressor is an N-terminally truncated form of the Spa2 protein important for polarized morphgenesis.4) A gene encoding Cch1, which has been genetically suggested to function with Mid1 as a Ca^<2+> channel, was cloned. We have confirmed that Mid1 and Cch1 really cooperate to function as a Ca^<2+> channel.5) Rice OsTpc1 that is homologous to yeast Cch1 was found to be sensitive to blockers of L-type voltage-gated calcium channels. In addition, The Arabidopsis Mca1 protein, which can complement the lethality of the yeast mid1 mutant, was isolated and characterized. Mca1 is crucial for Ca^<2+> influx and touch sensing in primary roots.

1)拉伸激活钙通道MID1的氨基酸残基Phe~(2+)与酵母细胞活力密切相关。系统地用各种氨基酸取代Phe^&lt；355&gt；表明该残基的疏水性是关键的。2)MID1蛋白定位于质膜和内质网膜。MID1通过二硫键形成寡聚体。3)分离并鉴定了MID1突变的多拷贝抑制因子。这种多拷贝抑制物是Spa2蛋白的N端截短形式，对极化形态发生很重要。4)克隆了编码Cch1的基因，该基因被认为与MID1一起作为一种钙离子通道发挥功能。5)与酵母Cch1同源的水稻OsTpc1对L类电压门控钙通道阻滞剂敏感。此外，还分离并鉴定了拟南芥Mca1蛋白，该蛋白可以补充酵母MID1突变体的致死性。Mca1是初级根中钙离子内流和触觉的关键因子。

项目成果

Identification of functional domains of Mid1, stretch-activated channel component, necessary for localization to the plasma membrane and Ca^<2+> permeation.

鉴定Mid1的功能域，拉伸激活的通道成分，对于定位到质膜和Ca ^ 2 渗透是必需的。

• 发表时间: 2005
• 期刊: Exp.Cell Res. 311
• 作者: Ozeki-Miyawaki;C.;Moriya;Y.;Tatsumi;H.;Iida H.;Sokabe;M.
• 通讯作者: M.

Phe356 in the yeast Ca2+ channel component Mid1 is a key residue for viability after exposeure to □-factor

酵母 Ca2+ 通道成分 Mid1 中的 Phe356 是暴露于 □ 因子后活力的关键残基

• 发表时间: 2004
• 期刊: Biochem. Biophs. Res. Commun 313
• 作者: Tada;T.;Ohmori;M.;Iida;H.
• 通讯作者: H.

Phe356 in the yeast Ca^<2+> channel component Mid1 is a key residue for viability after exposure to α-factor

酵母 Ca^<2+> 通道成分 Mid1 中的 Phe356 是暴露于 α 因子后活力的关键残基

• 发表时间: 2004
• 期刊: J.Biol.Chem. 313
• 作者: Tada;T.;Ohmori;M.;Iida;H.
• 通讯作者: H.

Arabidopsis plasma membrane protein crucial for Ca2+ influx and touch sensing in roots

• DOI: 10.1073/pnas.0607703104
• 发表时间: 2007-02-27
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Nakagawa, Yuko;Katagiri, Takeshi;Iida, Hidetoshi
• 通讯作者: Iida, Hidetoshi

Identification of functional domains of Mid1, a stretch-activated channel component, necessary for localization to the plasma membrane and Ca2+ permeation

• DOI: 10.1016/j.yexcr.2005.08.014
• 发表时间: 2005-11-15
• 期刊: EXPERIMENTAL CELL RESEARCH
• 影响因子: 3.7
• 作者: Ozeki-Miyawaki, C;Moriya, Y;Sokabe, M
• 通讯作者: Sokabe, M