Molecular Cell Biological Study on Calcium Signaling

钙信号传导的分子细胞生物学研究

• 批准号: 09680690
• 负责人: IIDA Hidetoshi
• 金额: $ 2.05万
• 依托单位: Tokyo Gakugei University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680690/
• 关键词: Calcium; Calcium signal; MID1 gene; Calcium channel; Budding yeast; Saccharomyces cerevisiae; Signal transduction; Transmembrane protein; カルシウムイオン; イオンチャネル; フェロモン; 分子細胞生物学

A goal of our research is to unravel molecular mechanisms of Ca^<2+> signaling during the mating process in the budding yeast Saccharomyces cerevisiae. The revious studies have revealed that the yeast MID1 gene product (Mid1) is required for calcium influx during the mating process, and found that Midi is a Ca^<2+>-permeable cation channel. In this study, we did a screen for mutations showing synthetic lethality with the mid1-1 allele. Synthetic lethality provides in vivo evidence that two gene products physically interact with each other or function in overlapping pathways. We could isolate forty synthetic lethal mutants and isolated a gene that conferred synthetic lethality. In another project, we made mutant midi genes, in which glutamate in the putative transmembrane H2 was substituted by other amino acids, and are now investigating channel acivities of these mutant Mid1 proteins. We also isolated seven midi mutant alleles, with the hope that we can identify amino acids important for the function of the Mid1 channel.

我们研究的目的是阐明在酿酒酵母萌芽过程中交配过程中Ca^<2+>信号传导的分子机制。复兴的研究表明，在交配过程中钙涌入需要酵母MID1基因产物（MID1），并发现MIDI是Ca^<2+> - 可渗透阳离子通道。在这项研究中，我们为突变进行了一个屏幕，显示了与中期1-1等位基因合成致死性的突变。合成致死性提供了体内证据，表明两种基因产物在重叠途径中彼此物理相互作用或功能。我们可以分离四十个合成致死突变体，并分离出赋予合成致死性的基因。在另一个项目中，我们制作了突变的MIDI基因，其中推定的跨膜H2中的谷氨酸被其他氨基酸取代，现在正在研究这些突变体MID1蛋白的通道效率。我们还分离了七个MIDI突变等位基因，希望我们可以鉴定出对MID1通道功能重要的氨基酸。

项目成果

Mori,I.C.: "Salicylic acid induces a cytosolic Ca^<2+> elevation in yeast" Biosci.Biotech.Biochem.(印刷中). (1998)

Mori，I.C.：“水杨酸诱导酵母中胞质Ca 2+ 升高”Biosci.Biotech.Biochem.（出版中）。

Mori,I.C.: "Salicylic acid induces a cytosolic Ca^<2+> elevation in yeast" Biosci.Biotechnol.Biochem.62. 986-989 (1998)

Mori,I.C.：“水杨酸诱导酵母中胞质Ca 2+ 升高”Biosci.Biotechnol.Biochem.62。

Mori, I.: "C.Salicylic acid induces a cytosolic Ca_<2+> elevation in yeast." Biosci.Biotechnol.Biochem.62. 986-989 (1998)

Mori, I.：“C.水杨酸诱导酵母细胞质 Ca_2 升高。”

Iida, H.: "Ca_<2+>-regulation of gene expression and cell division (In Japanese)" Protein, Nucleic Acid and Enzyme. 43. 1821-1828 (1998)

Iida, H.：“Ca_2-基因表达和细胞分裂的调节（日语）”蛋白质、核酸和酶。

飯田秀利: "Ca^<2+>による遺伝子発現と細胞分裂の制御" 蛋白質核酸酵素. 43. 1821-1828 (1998)

Hidetoshi Iida：“Ca ^ 2+ 对基因表达和细胞分裂的控制”蛋白质核酸酶。43。1821-1828（1998）