Studies on the regulation properties of CAM type photosynthesis in pineapple

菠萝CAM型光合作用调控特性的研究

• 批准号: 16380018
• 负责人: NOSE Akihiro
• 金额: $ 2.37万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380018/
• 关键词: CAM; pineapple; Kalancho*; PEP carboxylase; protein phosphorylation; CAM reguration; tonoplast; mitochondria; セイロンベンケイソウ; トノプラスト; リン脂質; タンパク比; 脂肪酸組成

Although CAM type CO_2 exchange in pineapple was partially regulated by the phosphorylation of phosphoenolpyruvate (PEP) carboxylase in night as observing in Kalanchoe CAM, the contribution to the CAM expression was less than the Kalanchoe CAM. It was appeared that the driving force of the malate accumulation on the tonoplast, such as ATPase and PPase, and the tonplast ligidity contributed to the CAM regulation. In addition, phosphofructokinase (PFP) of pineapple was constructed by subunits of α and β, the result showed that α subunit concerned to the PFP regulation as similar to other plant species. External NADH oxidation in mitochondria of pineapple, a typical PEP carboxykinase (PCK)-CAM plant, was more connected with the cytochrome pathway and less with the alternative pathway. This capacity might be one of the fitting mechanisms of pineapple to produce a sufficient amount of ATP for cytosolic PCK in the daytime. That is, it was suggested that the connection of electron transfer chain to cytochrome oxydase or alternative oxydase is different between the CAM subtypes of PCK and malic enzyme (ME). In summery, pineapple CAM type photosynthesis has a different regulation mechanism to the authentic CAM regulation observed in Kalanchoe specied, although some similar properties also contributed to both CAM regulations of pineapple and Kalanchoe. In the next, cross-talks of different metabolism and organs, such as glycolysis and mitochondria are important topics in the CAM regulation.

虽然菠萝CAM型CO_2交换在夜间部分受磷酸烯醇式丙酮酸（PEP）羧化酶的磷酸化调节，但其对CAM表达的贡献小于Kalamae CAM。这表明，苹果酸在液泡膜上积累的驱动力，如ATP酶和PPase，以及液泡膜的刚性有助于CAM的调节。此外，还将菠萝的磷酸果糖激酶（PFP）由α亚基和β亚基构建而成，结果表明α亚基与其他植物的PFP调控相似。菠萝是一种典型的PEP羧激酶（PCK）-CAM植物，其线粒体的外部NADH氧化更多地与细胞色素途径有关，而与旁路途径的联系较少。这种能力可能是菠萝在白天为胞质PCK产生足够量的ATP的合适机制之一。也就是说，这表明，电子转移链连接到细胞色素氧化酶或替代氧化酶是不同的CAM亚型的PCK和苹果酸酶（ME）。综上所述，菠萝CAM型光合作用具有不同的调节机制，以真正的CAM调节中观察到的Kalabe物种，虽然一些相似的属性也有助于菠萝和Kalabe的CAM调节。其次，不同代谢和器官之间的相互作用，如糖酵解和线粒体是CAM调控的重要课题。

项目成果

Reguratory properties of PEPC in three crassulacean acid metabolism (CAM) species, Ananas comosus, Kalancho* pinnata and K.daigremontiana : diurnal changes of PEPC phosphorylation state

PEPC 在三种景天酸代谢 (CAM) 物种（Ananas comosus、Kalancho* pinnata 和 K.daigremontiana）中的调节特性：PEPC 磷酸化状态的日变化

• 发表时间: 2005
• 期刊: Japanese Journal Crop Science 74(Extra issue 2)
• 作者: Vuthy Theng;Sakae Agarie;Akhir Nose
• 通讯作者: Akhir Nose

Effects of KCN, SHAM and oxygen concentrations on respiratory properties of purified mitochondria isolated from Ananas comosus (pineapple) and Kalancho* daigremontiana.

KCN、SHAM 和氧气浓度对从 Ananas comosus（菠萝）和 Kalancho* daigremontiana 中分离的纯化线粒体呼吸特性的影响。

• 发表时间: 2005
• 期刊: Plant Production Science 8(4)
• 作者: Hoang Thi Kim Hong;Akihiro Nose;Sakae Agarie
• 通讯作者: Sakae Agarie

Analysis of tonoplast lipid and protein composition in the two kinds of CAM plants, Ananas comosus and Kalancho* daigremontiana (In Japanese)

两种CAM植物Ananas comosus和Kalancho* daigremontiana的液泡膜脂质和蛋白质组成分析（日文）

• 发表时间: 2005
• 期刊: Japanese Journal Crop Science 74(Extra issue 2)
• 作者: Qin Lin;Yu-Ming Wang;Akihiro Nose;Hoang Thi Kim Hong;Sakae Agarie
• 通讯作者: Sakae Agarie

二種類のCAM植物(セイロンベンケイソウ,パインアップル)における液温変化が液胞膜の脂質とタンパクの構成成分に及ぼす影響

液体温度变化对两类CAM植物（锡兰景天和菠萝）液泡膜脂质和蛋白质成分的影响

• 发表时间: 2005
• 期刊: 日本作物学会紀事 74(別2)
• 作者: San-oh;Y.;大川泰一郎;M.Hirai;A.Kanno;S.Mori;A.Kanno;S.Mori;M.Hirai;A.Kanno;S.Mori;M.Nakada;M.Nakano;M.Nakano;M.Nakano;Y.Adachi;S.Mori;T.Nakamura;T.Nakamura;S.Mori;T.Nakamura;S.Mori;T.Ito;T.Nakamura;Y.Adachi;S.Mori;S.Mori;T.Ochiai;M.Nakano;M.Nakano;Qin Lin;Vuthy Theng;Hoang Thi Kim Hong;林 欽
• 通讯作者: 林 欽

Reguratory properties of PEPC in three crassulacean acid metabolism (CAM) species, Ananas comosus, Kalanchoe pinnata and K.daigremontiana : diurnal changes of PEPC phosphorylation state

PEPC 在三种景天酸代谢 (CAM) 物种（Ananas comosus、Kalanchoe pinnata 和 K.daigremontiana）中的调节特性：PEPC 磷酸化状态的日变化

• 发表时间: 2005
• 期刊: Japanese Journal Crop Science 74(Extra2)
• 作者: San-oh;Y.;大川泰一郎;M.Hirai;A.Kanno;S.Mori;A.Kanno;S.Mori;M.Hirai;A.Kanno;S.Mori;M.Nakada;M.Nakano;M.Nakano;M.Nakano;Y.Adachi;S.Mori;T.Nakamura;T.Nakamura;S.Mori;T.Nakamura;S.Mori;T.Ito;T.Nakamura;Y.Adachi;S.Mori;S.Mori;T.Ochiai;M.Nakano;M.Nakano;Qin Lin;Vuthy Theng;Hoang Thi Kim Hong;林 欽;Vuthy Theng
• 通讯作者: Vuthy Theng