Studies on regulating mechanisms of carbon distribution in rice lines of resistant and susceptible to sheath blight disease.

水稻抗纹枯病品系和感病品系碳分布调控机制研究.

• 批准号: 20580016
• 负责人: NOSE Akihiro
• 金额: $ 2.83万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580016/
• 关键词: イネ; 紋枯病; 炭素分配; 抵抗性; 感受性; 解糖系; QTL; 多系; 低温耐性; 根; ヘテロシス; フェニルプロパノイド代謝; 代謝中間体; 遺伝子発現

It was appeared that a resistance to the sheath blight disease (SBD) was resulted from a regulation in the carbon distribution named as a carbon crossroad inthe glycolysis, from the glycolysis to sucrose, starch and phenylpropanoid metabolisms, and also suggested that elucidations on the regulating mechanism become the next target ofmetabolic engineering studies to develop the SBD resistance. Also, the existence of twoQTLs in the SBD resistant rice line showed a possibility to improve resistant varieties. In addition, the gene linkage between SBD resistance and a heterosis of the yield in ourimproved SBD resistant rice line suggests a possibility to achieve both of the SBD resistance and the high yield.

这些结果表明，小麦对纹枯病的抗性是由于糖酵解过程中碳分配的一个重要调控过程，即糖酵解到蔗糖、淀粉和苯丙烷代谢过程中的碳十字路口，阐明其调控机制是小麦抗纹枯病代谢工程研究的下一个目标。同时，在抗SBD水稻品系中存在2个QTL，为抗病品种的改良提供了可能。此外，抗SBD水稻品系与产量杂种优势之间存在基因连锁，这提示了水稻既抗SBD又获得高产的可能性。

项目成果

Gene expression analysis of the mechanism of resistance to sheath blight fungus (Rhizoctonia solani) on selected rice lines using semi-quantitative RT-PCR

使用半定量 RT-PCR 对所选水稻品系抗纹枯病真菌（立枯丝核菌）的机制进行基因表达分析

• 发表时间: 2009
• 作者: Mutuku J.M.;Nose A
• 通讯作者: Nose A

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Gene expression analysis of the mechanism of resistance to sheath blight fungus(Rhizoctonia solani)on selected rice lines using semi-quantitative RTPCR

采用半定量 RTPCR 分析所选水稻品系抗纹枯病菌（立枯丝核菌）机制的基因表达

• 发表时间: 2009
• 作者: Mutuku J.M.;Nose A
• 通讯作者: Nose A

Rhizoctonia solani infection in two rice lines increases mRNA expression of metabolic enzyme genes in glycolytic, oxidative pentose phosphate pathways and secondary metabolism

两个水稻品系中立枯丝核菌感染增加了糖酵解、氧化戊糖磷酸途径和次生代谢中代谢酶基因的 mRNA 表达

• DOI: 10.11248/jsta.54.119
• 发表时间: 2010
• 期刊: Tropical agriculture and development
• 作者: M. Mutuku;A. Nose
• 通讯作者: A. Nose