Sub-critical Water Treatment of Wood Cellulose for Ethanol Production

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The research target of preprocessing technology development is using sub-critical water for directly employing to enzymatic saccharification modified insolubility in water wood cellulose. Results were as follows:(1) Development of wood fine-crusher: The fine-crusher consisted from frail type knives and rotary sieve was developed and defined as low power requirement. Machine design parameters were evaluated by using multivariate analysis for quantified index of fine-crushed wood of irregular pattern by introducing fractal dimension.(2) Lignin removal by sub-critical water; 1) Removal was investigated for various pattern and sizes of fine-crushed wood by changing temperatures and treatment times. Removal ratio was described by the function of fractal dimension of fine-crushed wood. 2) Removal ratio was defined depending on slurry concentration of fine-crushed wood in water. The ratio was decreased in higher concentration and constant over 4%.(3) Sub-critical water reformation of wood cellulose and enzymatic saccharification; Tissue paper and fine-crushed wood reformed by sub-critical water for various temperatures and treatment times were employed in enzymatic saccharification test by using Aspergillus niger and Tricoderma. The reformation effects were verified.