Involvement of the Wnt signaling-regulating factors APC and ICAT in cell differentiation and morphogenesis

Wnt信号调节因子APC和ICAT参与细胞分化和形态发生

• 批准号: 16390051
• 负责人: SENDA Takao
• 金额: $ 9.47万
• 依托单位: FUJITA HEALTH UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390051/
• 关键词: APC; PSD-95; post-synaptic spine; microtubules; Dig; ICAT; ureteric bud; アポトーシス; Wntシグナル系; DLG; 脳神経系; シナプス; 中腎管; 膣; 腎発生; 細胞増殖; 細胞接着; 培養神経細胞; AMPA受容体; クラスタリング

1. APC is required for the activity of AMPA receptors through facilitating the clustering of PSD-95 and these receptors at the post-synaptic spine.2. The APC localization in cultured neurons alters depending on their development and differentiation.3. Microtubule bundling-activity of APC is stimulated by its interaction with PSD-95.4. The kidneys and ureters of Dlghl-knockout mice were hypoplastic and the lower ends of the ureters were ectopic.5. The vagina and seminal vesicle were absent in the Dlghl-knockout mice. These phenotypes seem to be much alike to those in the human congenital disease Mayer-Rokitansky-Kuster-Hauser Syndrome.6. ICAT-knockout mice showed multiple developmental defects. Most of the ICAT(-/-) embryos exhibited a truncation of the rostral head.7. Some of the ICAT(-/-) embryos lacked kidneys. The defect in kidney development was found to be caused by arrest of the ureteric bud-branching during nephrogenesis.

1. APC通过促进PSD-95和这些受体在突触后棘的聚集，从而促进AMPA受体的活性。APC在培养神经元中的定位随神经元的发育和分化而改变。APC与PSD-95.4的相互作用刺激了APC的微管捆绑活性。dghl基因敲除小鼠的肾脏和输尿管发育不全，输尿管下端异位。dghl基因敲除小鼠没有阴道和精囊。这些表型似乎与人类先天性疾病梅耶-罗基坦斯基-库斯特-豪泽综合征非常相似。icat基因敲除小鼠出现多种发育缺陷。大多数ICAT（-/-）胚胎显示吻侧头截断。一些ICAT（-/-）胚胎缺少肾脏。肾脏发育的缺陷被发现是由于肾形成过程中输尿管芽分支的阻滞引起的。

项目成果

医療系学生のための解剖学実習

医学生解剖学实践

• 发表时间: 2005
• 作者: Shimomura A;Kohu K;Akiyama T;Senda T;Senda T;Senda T;Nomura R;Nakamura K;Shimomura A;Senda T;Iizuka-Kogo A;Senda T;Senda T;磯村源蔵
• 通讯作者: 磯村源蔵

Abnormal development of urogenital organs in Dlgh1-deficient mice

• DOI: 10.1242/dev.02830
• 发表时间: 2007-05-01
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Iizuka-Kogo, Akiko;Ishidao, Takefumi;Senda, Takao
• 通讯作者: Senda, Takao

Anti-caltrticulin antibody binds to a membrane protein in caveolae.

抗钙蛋白抗体与小窝中的膜蛋白结合。

• 发表时间: 2005
• 期刊: Acta Histochem Cytochem 38
• 作者: Shimomura A;Kohu K;Akiyama T;Senda T;Senda T;Senda T;Nomura R
• 通讯作者: Nomura R

Abnormal development of urogenital organs in Dlghl-deficient mice.

Dlghl 缺陷小鼠泌尿生殖器官发育异常。

• 发表时间: 2007
• 期刊: Development 134
• 作者: Kawasaki Y.;Sagara M.;Shibata S.;Shirouzu M.;Yokoyama S.;Akiyama T.;Murayama K.;Toya H.;Iizuka-Kogo A
• 通讯作者: Iizuka-Kogo A

Accumulation of BCL 10 at the perinuclear region is required for the BCL 10-mediated nuclear factor-κB Activation.

BCL 10 在核周区域的积累是 BCL 10 介导的核因子-κB 激活所必需的。

• 发表时间: 2005
• 期刊: Pathobiology 72
• 作者: Shimomura A;Kohu K;Akiyama T;Senda T;Senda T;Senda T;Nomura R;Nakamura K
• 通讯作者: Nakamura K