Analysis of mitochondria-nucleus inter-genetic network

我们这项研究的具体目的是阐明线粒体-细胞核遗传间网络系统。为了实现这一目的，我们建立了rho零杂交系统，其中细胞在线粒体tRNALeu（UUR）基因中具有不同百分比的A3243G突变。我们利用100%野生型和100%突变型的杂交体分离RNA并进行芯片分析，以确定线粒体-细胞核相互作用的候选控制基因。其中一个基因被鉴定为minitin，该基因被认为是一种癌症抑制基因，主要在食管癌中表达，是线粒体呼吸复合体I基因一个未确定的核亚基的候选基因。另一个项目是Klotho基因KO小鼠，这是公认的早期衰老和痴呆模型。我们尚未发现新的基因参与线粒体-细胞核遗传间网络系统。

Our specific aim of this study is to clarify the mitochondria-nucleus inter-genetic network system. To enable for this purpose, we established the rho zero cybrid system in which cell has various percentage of an A3243G mutation in the mitochondrial tRNALeu(UUR) gene. Using cybrids which has 100% wild type and 100% mutant, we isolated the RNA and analyzed microarray in order to identify the specific gene, a candidate of controlling gene of mitochondria-nucleus interaction. One gene was identified as minitin which was recognized as a cancer suppressing gene and expressed mainly in esophagus cancer, is a candidate for one of the unidentified nuclear subunits of mitochondrial respiratory complex I gene. Another project is the Klotho gene KO mouse, which is recognized as early senile and dementic model We have not been identified of new gene which contribute the mitochondria-nucleus inter-genetic network system.