DISC REPAIR WITH HUMAN CELL, THERAPYINFLUENCED BY MESENCHYMAL STEM CELLS

由于髓核细胞数量少，生物学活性低，因此有必要开发新的活化方法或寻找新的细胞来源来替代退化的髓核细胞。利用动物模型，细胞数量和DNA和蛋白多糖的合成数据表明，髓核细胞与骨髓间充质干细胞具有直接细胞间接触的共培养系统被有效地激活。使用椎间盘退变动物模型（如比格犬）的体内研究也显示，可减缓椎间盘退变或再生的进展速度。对于活化的髓核细胞的人应用，还评价了通过具有直接细胞间接触的共培养的人间充质干细胞对人髓核细胞的活化。DNA和蛋白多糖的合成比单层培养高5倍。未发现染色体异常。未观察到肿瘤发生。因此，我们认为活化的髓核细胞是非常有用和安全的临床应用。

Since only a very small number of nucleus pulposus cells could be obtained and their biological activity was low, it is necessary to develop a new activation method or find a new source of cells to replace the degenerated NP cells. Utilizing animal models, the data on the number of cells and the synthesis of DNA and proteoglycans showed that the nucleus pulposus cells were effectively activated by coculture system with bone marrow-derived mesenchymal stem cell having direct cell-to-cell contact. In vivo study using animal models such as beagle dog with disc degeneration also revealed to decelerate the velocity of progression of disc degeneration or regeneration. For the human application of the activated nucleus pulposus cells, an activation of human nucleus pulposus cells by human mesenchymal stem cells with coculture having direct cell-to-cell contact was also evaluated. The synthesis of DNA and proteoglycan revealed 5- fold higher than in the monolayer culture. No abnormality on the chromosome was revealed. Tumor genesis was not observed. Therefore, we concluded that the activated nucleus pulposus cells are very useful and safe for clinical application.