Disc repair with transplantation of activated nucleus pulposus cells-how to preserve the cells and its safety-

本研究的目的是评价冷冻保存后活化的髓核细胞的安全性和性能。以动物模型和人体材料为实验材料，比较两种冻存方法：1)冷冻保存2周后活化的髓核细胞，2)冷冻保存2周后的髓核组织本身，比较两组正常激活的髓核细胞的安全性和性能。这三个亚组在安全性、髓核细胞的增殖以及在动物和人类材料中形成细胞基质的潜力方面没有显著差异。我们证实了髓核的冷冻保存对修复各种椎间盘损伤是有用的。

The purpose of this study is to evaluate the safety and property of the activated nucleus pulposus cells after the cryopreservation. With the animal model and human material, we compared safety and property of the nucleus pulposus cells, ordinarily activated, to those in two subgroups on cryopreservation method ; 1) activated nucleus pulposus cells after cryopreservation for two weeks and 2) nucleus pulposus tissue itself after cryopreservation for two weeks. There was no significant difference amongst those three subgroups on the safety, the proliferation of the nucleus pulposus cells or the potentiality for the cell matrix in both animal and human materials. We confirmed cryopreservation of the nucleus pulposus is useful to repair various disc lesions.