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Study on tissue regeneration using stem cells from a fetus belonging

利用胎儿干细胞进行组织再生的研究

基本信息

批准号：
16390508
负责人：
HIROTAKA Asato
金额：
$ 8.9万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390508/
关键词：
fetus belonging umbilical cord Wharton's jelly mesenchymal stem cells human umbilical cord endothelial cells co-implantation matrigel neovascularization 臍帯静脈内皮細胞臍帯間質細胞

项目摘要

We examined about establishment of collection methods of useful cells from the umbilical cord, which was disposal biomaterials, and application to the reproduction medicine. We used 0.25% trypsin /EDTA current method to umbilical cord vein and gathered Human umbilical cord vein cell(HUVEC). We used explant method to a Wharton' s jelly and gathered Human umbilical stromal cell (HUSC). In FACS analysis, our HUVEC have an approximately equal surface marker (CD31+, CD144+, Flk-1+) in comparison with commercial HUVEC. Our HUVEC also uptake acLDL, which is a basic property of an endothelium. On the other hand, HUSC was able to differentiation to cartilage, fat, bone. In FACS analysis, cells from frozen umbilical cord block have an approximately equal surface marker in comparison with cells from fresh umbilical cord block. In Matrigel assay, HUSC alone did not build network such as HUVEC, but was able to commit in a network in HUVEC. In ischemic limbs model, co-injection of HUVEC and HUSC can rescue ischemic limbs. It was thought that a neovascularization was indespensable to reproduction of the internal organs in current reproduction medicine. In the near future, using HUVEC and HUSC together well was thought to rebuild the internal organs.

探讨了脐带有用细胞采集方法的建立及其在生殖医学中的应用。采用0.25%胰酶/EDTA电流法采集人脐静脉内皮细胞(HUVEC)。采用组织块法对沃顿氏S凝胶进行体外培养，收集人脐血间充质细胞。在FACS分析中，我们的人脐静脉内皮细胞与商品化的人脐静脉内皮细胞相比，具有大致相同的表面标志(CD31+，CD144+，Flk-1+)。我们的HUVEC也摄取acLDL，这是内皮的一个基本属性。另一方面，HUSC可向软骨、脂肪、骨等方向分化。在FACS分析中，冷冻脐带块细胞与新鲜脐带块细胞的表面标记大致相同。在Matrigel实验中，单独的HUSC没有构建HUVEC这样的网络，但能够在HUVEC中建立网络。在肢体缺血模型中，联合注射HUVEC和HUSC可以挽救缺血肢体。在目前的生殖医学中，血管新生对于内脏器官的再生是不可忽视的。在不久的将来，将HUVEC和HUSC很好地结合起来被认为可以重建内脏器官。