Studies on Secondary Caries Initiation Mechanism and Finding Prevention Methods Using a Newly Modified Oral Biofilm Reactor

使用新型口腔生物膜反应器研究继发龋发生机制并寻找预防方法

• 批准号: 16390544
• 负责人: MATIN Khairul
• 金额: $ 8.96万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390544/
• 关键词: Primary caries; Secondary caries; Cariogenic Bacteria; Oral Biofflm Reactor (QBE); Arifieial biofilm; Self-surface-cleaning; Glucan; 2-DE; 人工口腔装置; 人工バイオフィルム; グルカン; アルカリイオン水; オゾン; Mass Spectrometry; biofilm; Artificial Mouth System(AMS); mu

Newly modified computer software (EPC-2000) operated artificial mouth system (AMS) was developed and renamed as oral biofilm reactor (OBR). Automatic delivery of bacterial suspensions and mediums on to specimens became possible by controlling the flow rate in accordance of the need of a particular study simulating the human oral environments.Biofilm induced caries models, namely; 1) Primary caries model or White Spot Enamel Lesion (WSEL) and 2) Secondary caries model were established using bovine and human extracted teeth. Visible WSELs were examined by Quantitative light-induced fluorescence (QLF), scanning electron microscope (SEM), energy dispersive X-ray spectroscope (EDS) and nanoindentor. Results indicated that demineralization occurred on the enamel surfaces. The lesion depth increased time dependently that showed co-relationship with Ca and P mineral loss and loss of nano-hardeness and elasticity. The artificial secondary caries induced by multi-species cariogenic bacteria at the interface of composite restoration showed wide gap-formation when bonding material was not used. Quantitative genomic DNA analysis (Real-time PCR) confirmed invasion of bacteria in the demineralized lesion and lesions were non-invasively indentified by Micro-CT.Newly developed dental materials (including adhesives, fluoride oligomer incorporated coating, Teflon incorporated restoratives) were investigated for their self-surface-cleaning capability and biofilm inhibition. Also, alkali-ion water (AW) found to be effective in dissolving glucan polymers of the biofilms. Real-time PCR and 2-DE showed variations on DNA quantification and protein expression after AW or ozone treatment.

开发了新修改的计算机软件（EPC-2000）操作的人工口腔系统（AMS）并更名为口腔生物膜反应器（OBR）。通过根据模拟人类口腔环境的特定研究的需要控制流速，可以将细菌悬浮液和培养基自动输送到样本上。生物膜诱导龋齿模型，即； 1) 原发性龋齿模型或白斑牙釉质病变 (WSEL) 和 2) 使用牛和人拔牙建立继发性龋齿模型。通过定量光诱导荧光 (QLF)、扫描电子显微镜 (SEM)、能量色散 X 射线光谱仪 (EDS) 和纳米压痕仪检查可见 WSEL。结果表明，牙釉质表面发生脱矿质。损伤深度随着时间的推移而增加，这表明与 Ca 和 P 矿物质损失以及纳米硬度和弹性损失存在相关性。当不使用粘结材料时，复合修复体界面处由多种致龋菌诱导的人工继发龋表现出宽间隙形成。定量基因组 DNA 分析（实时 PCR）证实脱矿病灶中存在细菌入侵，并通过 Micro-CT 非侵入性地识别病灶。研究了新开发的牙科材料（包括粘合剂、氟化物低聚物掺入涂层、聚四氟乙烯掺入修复剂）的自表面清洁能力和生物膜抑制能力。此外，碱离子水（AW）被发现能有效溶解生物膜的葡聚糖聚合物。实时 PCR 和 2-DE 显示 AW 或臭氧处理后 DNA 定量和蛋白质表达的变化。

项目成果

アルカリイオン水(AW)のバイォフィルム除去促進効果

碱性离子水（AW）的生物膜去除促进效果

• 发表时间: 2005

An ingenious design for peptide vaccines

• DOI: 10.1016/j.vaccine.2005.01.031
• 发表时间: 2005-03-18
• 期刊: VACCINE
• 影响因子: 5.5
• 作者: Yano, A;Onozuka, A;Nisizawa, T
• 通讯作者: Nisizawa, T

Quantitative assessment for stimulated saliva flow rate and buffering capacity in relation to different ages

• DOI: 10.1016/j.jdent.2006.01.004
• 发表时间: 2006-10-01
• 期刊: JOURNAL OF DENTISTRY
• 影响因子: 4.4
• 作者: Moritsuka, Michiyo;Kitasako, Yuichi;Nomura, Satoshi
• 通讯作者: Nomura, Satoshi

Storage media to preserve dentin and their effects on surface properties

保存牙本质的存储介质及其对表面特性的影响

• 发表时间: 2006
• 期刊: International Chinese Journal of Dentistry 6
• 作者: Shamim S;Nikaido T;AsafujjohaMd;Tagami J and Matin K.
• 通讯作者: Tagami J and Matin K.

Storage media to preserve dentin and their effects on surface properties.

保存牙本质的存储介质及其对表面特性的影响。

• 发表时间: 2006
• 期刊: Int Chin J Dent 6
• 作者: Shamim S;Nikaido T;Asafujjoha Md;Tagami J;Matin K.
• 通讯作者: Matin K.