Molecular and cell biological approach to promote the eruption of impacted teeth

促进阻生牙萌出的分子和细胞生物学方法

• 批准号: 16390604
• 负责人: SUDA Naoto
• 金额: $ 8万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390604/
• 关键词: tooth impaction; delayed tooth eruption; drug delivery system; formation of eruption pathway; gene analysis; DNA microarray; 埋伏歯

Gene expression regarding the tooth expression was examined in this research project to develop a novel non-invasive treatment for tooth impaction and delayed tooth eruption. The following are research results of this year.1) Genes which were significantly up-regulated and down-regulated by the tooth eruption were clarified by using DNA microarray2) Periodontal ligament cells were isolated from the patient with Marfan syndrome that has mutation in the gene encoding fibrillin 1. Teromerase reverse transcriptase and Bmi-1 were introduced into these cells by using retrovirus.3) ADMTSL-1 was discovered to associate with fibrillin 1.4) In vivo system, in which root resorption was induced reproducibly, was established. In this system, the mandibular molars were extracted and reimplanted to SCID mice after UV irradiation and/or air dry.5) DNA chip, Perio gen chip, was developed. This is based on the gene expression profile of human periodontal ligament cells.6) The inhibitory effect of Amelogenin known as bioactive substrate of EMDOGAIN for tooth root resorption was examined. Amelogenin was found to have inhibitory effect on odontoclastic root resorption.7) This inhibitory effect was via suppressing RANKL expression.

本研究旨在通过检测与牙齿表达相关的基因表达，开发一种新的非侵入性治疗牙齿嵌塞和牙齿延迟萌出的方法。本年度的研究结果如下：1）通过DNA微阵列，明确了伴随牙齿萌出而显著上调和下调的基因2）从编码牙周膜蛋白1的基因发生突变的马凡氏综合征患者中分离出牙周膜韧带细胞。3）ADMTSL-1可与牙髓细胞结合。4）建立了可重复诱导牙根吸收的体内实验系统。5）制备了DNA芯片，即Perio gen芯片。这是基于人牙周膜细胞的基因表达谱。6）检查了作为EMDOGAIN的生物活性底物的釉原蛋白对牙根吸收的抑制作用。发现釉原蛋白对破牙性牙根吸收具有抑制作用。7）这种抑制作用是通过抑制RANKL表达。

项目成果

A case of oculo-auriculo vertebral spectrum with longterm and comprehensive treatment.

眼耳椎谱系长期综合治疗一例

• 期刊: World Journal of Orthodontics (印刷中)
• 作者: Suda N;Watanabe M;Ohyama K
• 通讯作者: Ohyama K

Orthodontic treatment in a case of Van Woude syndrome.

Van Woude 综合征病例的正畸治疗。

• 期刊: Am J Orthod Dentofacial Orthop (in press)
• 作者: Suda N;Takada J;Ohyama K.
• 通讯作者: Ohyama K.

A case of amelogenesis imperfecta, cleft lip and palate and polycystic kidney disease.

• DOI: 10.1111/j.1601-6343.2006.00337.x
• 发表时间: 2006-02-01
• 期刊: Orthodontics & craniofacial research
• 影响因子: 3.1
• 作者: Suda, N;Kitahara, Y;Ohyama, K
• 通讯作者: Ohyama, K

Runx2-deficient mice lack mandibular condylar cartilage and have deformed Meckel's cartilage

• DOI: 10.1007/s00429-004-0393-2
• 发表时间: 2004-07-01
• 期刊: ANATOMY AND EMBRYOLOGY
• 作者: Shibata, S;Suda, N;Komori, T
• 通讯作者: Komori, T

Accelerated bone formation and increased osteoblast number contribute to the abnormalttooth germ development in prathyroid hormone-related protein (PTHrP)-knockout mice.

甲状旁腺激素相关蛋白（PTHrP）敲除小鼠中，骨形成加速和成骨细胞数量增加导致牙胚发育异常。

• 发表时间: 2004
• 期刊: Bone 35・5
• 作者: Kitahara Y;Suda N;Terashima T;Mekaapiruk K;Hammond VE;Takano Y;Ohyama K
• 通讯作者: Ohyama K