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Regeneration of resorbed tooth roots by cementogenesis

通过牙骨质形成再生吸收的牙根

基本信息

批准号：
18390552
负责人：
SUDA Naoto
金额：
$ 11.01万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390552/
关键词：
root resorption cementoblast regeneration amelogenin emdogain odontoclast 成長因子破歯細胞

项目摘要

Pathological tooth root resorption is frequently seen with infection, trauma and tumor, and also induced by some dental treatments. Prevention of pathological resorption in Permanent teeth is a crucial issue to avoid tooth loss and maintain normal oral function, however, there is no effective approach or management for it, so far.Emdogain gelR is a porcine enamel matrix derivative which can promote periodontal tissue regeneration. Since amelogenin is known as one of the bioactive substances in Emdogain, it is quite interesting to ask whether amelogenin and Emdogain have an inhibitory action on odontoclastic root resorption. To clarify this point; we examined the effect of amelogenin on odontoclast formation, in vitro. Furthermore, rat molars were replanted and the root resorption was induced. Emdogain was treated and the effect, on root resorption was examined, in vivo.Odontoclastic cells were isolated from human deciduous teeth and cultured in the presence of 1α25(OH)2vitaminD3 (Vit. D3). The upper first molars off 6 week-old male rats were extracted, and teeth were left on the bench for 1 hr after periodontal ligaments were removed Each tooth was replanted into the socket Root surfaces of some teeth were treated with Emdogain gelR or emdogain powder mixed with the propylene glycol alginate before the replantation. Rats were sacrificed 7 days after replantation and serial saggital sections were prepared.1) P172 treatment significantly decreased odontoclastic Dell number in the culture.2) Numerous root resorption lacunae and odontoclasts were seen on the root surface of replanted teeth. In the experimental group, in which Emdogain gelR or Emdogain powder was applied on roots, values of the odontoclast surface, odontoclast number, and the resorbed dentin and cementum were all significantly lower than those of untreated roots.As conclusion, all these findings suggest that amelogenin and Emdogain inhibit the root resorption, in vitro and in vivo.

病理性牙根吸收常见于感染、外伤和肿瘤，也可由某些牙科治疗引起。预防恒牙病理性吸收是避免牙齿脱落和维持正常口腔功能的关键问题，但目前尚无有效的方法和管理方法。Emdogain gelR是一种促进牙周组织再生的猪牙釉质基质衍生物。由于淀粉原蛋白被认为是Emdogain中的生物活性物质之一，所以淀粉原蛋白和Emdogain是否对破牙根吸收有抑制作用是一个非常有趣的问题。为了澄清这一点；我们在体外研究了淀粉原蛋白对破牙细胞形成的影响。再植大鼠磨牙，诱导牙根吸收。用Emdogain处理后，在体内观察其对牙根吸收的影响。从人乳牙中分离出破牙细胞，在1α25(OH)2vitaminD3 （Vit）存在下培养。D3)。取6周龄雄性大鼠上颌第一磨牙，拔除牙周韧带后静置1小时，每颗牙在牙槽内再植，部分牙根面用Emdogain凝胶或Emdogain粉与海藻酸丙二醇混合后再植。大鼠于再植后第7天处死，制作连续矢状面切片。1) P172处理显著降低了培养体中牙髓破胶细胞的数量。2)再植牙根表面可见大量的根吸收腔隙和破牙细胞。实验组牙根涂抹Emdogain凝胶或Emdogain粉末后，破牙细胞表面积、破牙细胞数量、牙本质和牙骨质的吸收均显著低于未处理组。综上所述，在体内和体外实验中，淀粉原蛋白和Emdogain均能抑制根吸收。