Visualization of osteocyte differentiation in living bone

活骨中骨细胞分化的可视化

• 批准号: 16390606
• 负责人: KAMIOKA Hiroshi
• 金额: $ 9.34万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390606/
• 关键词: osteocytes; osteoblasts; 3D reconstruction; bone; actin filament

First, we could succeeded to reconstruct osteocyte network in bone using IMARIS, NEURON TRACER, SURPASS software. We informed the results in international journal "BONE" in 2005. Precise information is in the paper. Briefly, we measured osteocyte morphometry in chick calvaria and also showed morphological differences between osteocytes and osteoblasts. This technique was applied to visualize living osteocytes in bone by using OB7.3 chick osteocyte specific antibody. Now, we are in the way to visualize osteoblasts and osteocytes in living bone.Besides the morphological differences between osteoblasts and osteocytes, we for the first time demonstrated significant difference between the cells in response to mechanical stress, especially in fluid shear stress. In the study, osteocytes were less respositive to fluid shear stress than osteoblasts. It was believed that osteocytes are the chief mechanosensory cells in bone.However, in our knowledge based on the present study, osteocyte showed less calcium response than osteoblasts. In addition, we elucidated the implication of different focal adhesion formation between the cells.Therefore, we now showed morphological and functional differences in osteocytes by the grant this time.

首先，我们使用IMARIS、NEURON TRACER、SURPASS软件成功地重建了骨中的骨细胞网络。我们于2005年在国际期刊《BONE》上公布了结果。准确的信息在论文中。简而言之，我们测量了鸡颅骨中的骨细胞形态测量，并显示了骨细胞和成骨细胞之间的形态差异。该技术通过使用 OB7.3 小鸡骨细胞特异性抗体来可视化骨中的活骨细胞。现在，我们正在努力观察活骨中的成骨细胞和骨细胞。除了成骨细胞和骨细胞之间的形态差异外，我们还首次证明了细胞之间对机械应力（尤其是流体剪切应力）的响应存在显着差异。在这项研究中，骨细胞对流体剪切应力的反应不如成骨细胞。人们认为骨细胞是骨骼中主要的机械感觉细胞。然而，根据我们基于本研究的知识，骨细胞表现出比成骨细胞更少的钙反应。此外，我们阐明了细胞之间不同的粘着斑形成的含义。因此，我们这次通过资助展示了骨细胞的形态和功能差异。

项目成果

Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: Implication of different focal adhesion formation

• DOI: 10.1359/jbmr.060408
• 发表时间: 2006-07-01
• 期刊: JOURNAL OF BONE AND MINERAL RESEARCH
• 影响因子: 6.2
• 作者: Kamioka, Hiroshi;Sugawara, Yasuyo;Takano-Yamamoto, Teruko
• 通讯作者: Takano-Yamamoto, Teruko

Three-dimensional reconstruction of chick calvarial osteocytes and their cell processes using confocal microscopy.

• DOI: 10.1016/j.bone.2004.10.008
• 发表时间: 2005-05
• 期刊: Bone
• 影响因子: 4.1
• 作者: Yasuyo Sugawara;H. Kamioka;T. Honjo;Ken-Ichi Tezuka;T. Takano-Yamamoto