Periodontal regeneration by BMP application to root surface and scaffold of periodontal ligament

BMP应用于牙根表面和牙周膜支架的牙周再生

基本信息

批准号：
16390610
负责人：
SUGAYA Tsutomu
金额：
$ 6.29万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390610/
关键词：
Periodontal regeneration BMP-2 Collagen hydrogel Root surface conditioning Scaffold Hard tissue formation Root resorption Coupling コラーゲン 1壁性骨欠損

项目摘要

The aim of the first experiment was to evaluate the effect of different dentin conditionings followed rhBMP-2 application on dentin surface. Dentin blocks were demineralized with EDTA, tetracycline-HCI or citric acid solutions for 3 minutes, and were soaked with rhBMP-2 solution. Periodontal ligament cells were seeded on each dentin blocks, and ALP activity of attachment cells were assessed. The results showed that the groups of EDTA demineralization had higher ALP activity. In the second experiment, each dentin blocks were transplanted into palatal connective tissue of rats. The histological examinations showed that the groups of EDTA demineralization and rhBMP-2 application had significantly more hard tissue formation than any other groups. In the third experiment, dentin blocks were demineralized with EDTA and rhBMP-2 applied, and then implanted into palatal connective tissue of rats. Bisphosponate was administered to rats for osteoclast suppression. The results showed that hard tis … More sue formation and dentin resorption were decreased. It suggested that coupling system between osteoblast and osteoclast may play an important role in hard tissue formation on dentin surface. The aim of fourth study was to evaluate periodontal regeneration in dehiscence model after EDTA demineralization, application of BMP-2 solution and collagen hydrogel to root dentin surfaces. New bone formation was significantly greater than any other group. Then, periodontal regeneration in 1-wall infrabony defects was evaluated. New bone and new cementum formation was significantly greater than in the control group. New cementum isolated from original cementum was frequently observed in the coronal area, and it might be formed by gingival connective tissue. These results suggest that new aspects of the regenerative process are introduced.It was concluded that the application of BMP-2 solution and collagen hydrogel to root dentin surface was conducive to periodontal tissue regeneration. This procedure may be effective regenerative therapy for large periodontal defects. Less

第一个实验的目的是评估RHBMP-2在牙本质表面应用后，评估不同牙本质条件的影响。用EDTA，四环素HCI或柠檬酸溶液将牙本质块脱矿3分钟，并用RHBMP-2溶液浸泡。将牙周韧带细胞播种在每个牙本质块上，并评估附着细胞的ALP活性。结果表明，EDTA脱矿化组具有较高的ALP活性。在第二个实验中，将每个牙本质块移植到大鼠的pal骨连接的组织中。组织学检查表明，EDTA脱矿化和RHBMP-2的施用组的硬组织形成明显高于任何其他组。在第三个实验中，牙本质块用EDTA和RHBMP-2偿还了债务，然后植入大鼠的pal骨连接组织中。将双反发酸盐用于大鼠以进行破骨细胞抑制。结果表明，艰苦的tis…更多的sue形成和牙本质分辨率得到了改善。它表明，成骨细胞和破骨细胞之间的耦合系统可能在牙本质表面的硬组织形成中起重要作用。第四项研究的目的是评估EDTA债务化，BMP-2溶液和胶原水凝胶在根牙本质表面上的应用后，在脱脂模型中评估牙周再生。新的骨形成のです。英语：新的骨骼和新的骨质形成明显大于任何其他群体。然后，评估了1壁基raby不足的牙周再生。新的骨骼和新的骨质形成明显大于对照组。在冠状区域经常观察到从原始胶质中分离出来的新胶质，并且可能由牙龈结缔组织形成。这些结果表明，引入了再生过程的新方面。得出的结论是，BMP-2溶液和胶原水凝胶在根牙本质表面的应用正在进行牙周组织再生。对于大牙周缺损，该程序可能是有效的再生疗法。较少的