Periodontal regeneration by BMP application to root surface and scaffold of periodontal ligament

第一个实验的目的是评价在牙本质表面应用重组人骨形态发生蛋白-2后不同的牙本质条件对牙本质的影响。牙本质块用EDTA、四环素-盐酸或柠檬酸溶液脱矿3min，再用重组人骨形态发生蛋白-2溶液浸泡。在每个牙本质块上种植牙周膜细胞，并检测附着细胞的碱性磷酸酶活性。结果表明，EDTA脱矿组的ALP活性较高。实验二，将每个牙本质块移植到大鼠的腭部结缔组织中。组织学检查显示，EDTA脱矿和rhBMP-2应用组的硬组织形成明显多于其他组。实验三：应用EDTA和rhBMP-2脱矿，将牙本质块植入大鼠腭部结缔组织内。双磷酸盐对大鼠破骨细胞有抑制作用。结果表明，硬质TIS…更多的牙周组织形成和牙本质吸收减少。提示成骨细胞与破骨细胞之间的偶联系统在牙本质表面硬组织形成过程中可能起重要作用。第四项研究的目的是评价EDTA脱矿、BMP-2溶液和胶原水凝胶在根面牙本质表面应用后裂开模型的牙周再生。新骨形成显著多于任何其他组。然后，对1壁骨下骨缺损的牙周再生进行评估。新骨和新牙骨质的形成明显多于对照组。冠区常可见从原牙骨质中分离出的新牙骨质，可能由牙周结缔组织形成。结论：BMP-2溶液和胶原水凝胶应用于根面牙本质表面有利于牙周组织的再生。该方法有可能成为治疗大型牙周缺损的有效再生治疗方法。较少

The aim of the first experiment was to evaluate the effect of different dentin conditionings followed rhBMP-2 application on dentin surface. Dentin blocks were demineralized with EDTA, tetracycline-HCI or citric acid solutions for 3 minutes, and were soaked with rhBMP-2 solution. Periodontal ligament cells were seeded on each dentin blocks, and ALP activity of attachment cells were assessed. The results showed that the groups of EDTA demineralization had higher ALP activity. In the second experiment, each dentin blocks were transplanted into palatal connective tissue of rats. The histological examinations showed that the groups of EDTA demineralization and rhBMP-2 application had significantly more hard tissue formation than any other groups. In the third experiment, dentin blocks were demineralized with EDTA and rhBMP-2 applied, and then implanted into palatal connective tissue of rats. Bisphosponate was administered to rats for osteoclast suppression. The results showed that hard tis … More sue formation and dentin resorption were decreased. It suggested that coupling system between osteoblast and osteoclast may play an important role in hard tissue formation on dentin surface. The aim of fourth study was to evaluate periodontal regeneration in dehiscence model after EDTA demineralization, application of BMP-2 solution and collagen hydrogel to root dentin surfaces. New bone formation was significantly greater than any other group. Then, periodontal regeneration in 1-wall infrabony defects was evaluated. New bone and new cementum formation was significantly greater than in the control group. New cementum isolated from original cementum was frequently observed in the coronal area, and it might be formed by gingival connective tissue. These results suggest that new aspects of the regenerative process are introduced.It was concluded that the application of BMP-2 solution and collagen hydrogel to root dentin surface was conducive to periodontal tissue regeneration. This procedure may be effective regenerative therapy for large periodontal defects. Less