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Periodontal regeneration by BMP application to root surface and scaffold of periodontal ligament

BMP应用于牙根表面和牙周膜支架的牙周再生

基本信息

批准号：
16390610
负责人：
SUGAYA Tsutomu
金额：
$ 6.29万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390610/
关键词：
Periodontal regeneration BMP-2 Collagen hydrogel Root surface conditioning Scaffold Hard tissue formation Root resorption Coupling コラーゲン 1壁性骨欠損

项目摘要

The aim of the first experiment was to evaluate the effect of different dentin conditionings followed rhBMP-2 application on dentin surface. Dentin blocks were demineralized with EDTA, tetracycline-HCI or citric acid solutions for 3 minutes, and were soaked with rhBMP-2 solution. Periodontal ligament cells were seeded on each dentin blocks, and ALP activity of attachment cells were assessed. The results showed that the groups of EDTA demineralization had higher ALP activity. In the second experiment, each dentin blocks were transplanted into palatal connective tissue of rats. The histological examinations showed that the groups of EDTA demineralization and rhBMP-2 application had significantly more hard tissue formation than any other groups. In the third experiment, dentin blocks were demineralized with EDTA and rhBMP-2 applied, and then implanted into palatal connective tissue of rats. Bisphosponate was administered to rats for osteoclast suppression. The results showed that hard tis … More sue formation and dentin resorption were decreased. It suggested that coupling system between osteoblast and osteoclast may play an important role in hard tissue formation on dentin surface. The aim of fourth study was to evaluate periodontal regeneration in dehiscence model after EDTA demineralization, application of BMP-2 solution and collagen hydrogel to root dentin surfaces. New bone formation was significantly greater than any other group. Then, periodontal regeneration in 1-wall infrabony defects was evaluated. New bone and new cementum formation was significantly greater than in the control group. New cementum isolated from original cementum was frequently observed in the coronal area, and it might be formed by gingival connective tissue. These results suggest that new aspects of the regenerative process are introduced.It was concluded that the application of BMP-2 solution and collagen hydrogel to root dentin surface was conducive to periodontal tissue regeneration. This procedure may be effective regenerative therapy for large periodontal defects. Less

第一个实验的目的是评价rhBMP-2应用于牙本质表面后不同牙本质处理的效果。牙本质块用EDTA、盐酸四环素或柠檬酸溶液脱矿3分钟，并用rhBMP-2溶液浸泡。牙周膜韧带细胞接种在每个牙本质块上，并评估附着细胞的ALP活性。结果表明，EDTA脱矿组ALP活性较高。实验二将各牙本质块移植到大鼠腭部结缔组织中。组织学检查显示，EDTA脱矿组和rhBMP-2组的硬组织形成明显多于其他组。实验三：将牙本质块用EDTA脱矿，rhBMP-2处理后植入大鼠腭部结缔组织中。对大鼠给予双磷酸盐以抑制破骨细胞。结果表明，硬组织 ...更多信息牙本质吸收减少。提示成骨细胞和破骨细胞之间的偶联系统在牙本质表面硬组织形成中起重要作用。第四项研究的目的是评价EDTA脱矿后裂开模型中的牙周再生，BMP-2溶液和胶原水凝胶应用于根牙本质表面。新骨形成显著大于任何其他组。然后，牙周再生在单壁骨下缺损进行了评价。新骨和新牙骨质形成明显大于对照组。在冠区常可见从原有牙骨质中分离出来的新牙骨质，可能由牙龈结缔组织形成。这些结果表明，BMP-2溶液和胶原水凝胶应用于根面牙本质表面有利于牙周组织再生。该手术可能是大面积牙周缺损的有效再生治疗方法。少