婦人科悪性腫瘍におけるEtsファミリーの発現と機能の解析

Ets家族在妇科恶性肿瘤中的表达及功能分析

• 批准号: 16790961
• 负责人: 高井 教行
• 金额: $ 2.3万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16790961/
• 关键词: 子宮頚癌; 子宮体癌; 卵巣癌; c-Ets1; Elf-1; 浸潤; 転移

ProtooncogeneのEtsファミリーには、標的になる遺伝子・蛋白としてuPA, MMP-1,MMP-3,MMP-9,integrinなどの蛋白分解酵素、cyclinファミリ-などの細胞周期調節蛋白などがあり、癌の浸潤・転移や増殖・分化に関与する。われわれはEts-1蛋白が子宮体癌[Takai et al., Cancer,2000]と上皮性卵巣癌[Takai et al., Int J Mol Med,2002]において過剰発現し、Elf-1蛋白の発現が子宮体癌[Takai et al., Gynecol Oncol,2003]と上皮性卵巣癌[Takai et al., Int J Mol Med,2003]の病理組織学的予後因子と相関していることを報告してきた。しかし、この2つを含むすべてのEtsファミリーのmRNAや蛋白が臨床的にどのような癌細胞で、どのような分化度の細胞で、どの程度の発現が認められるのかなどほとんど解析されていない。そこで、子宮癌、卵巣癌の増殖・分化・浸潤・転移機構とEtsファミリーとの関係について検討し、さらにその癌に罹患した患者の予後を検討した。すなわちEtsファミリーが、どうの様な癌細胞で、どのような分化度の細胞で、どの程度の発現が認められるのかを確認した。さらに、臨床材料では解析不可能なmitogenなどによる刺激実験を培養癌細胞(HeLa細胞、HUAA細胞、OMC-3細胞など)で解析を行った。ノーザン法、in situ hybridization法、免疫組織化学法により、Etsファミリーは分化度の低い症例や臨床進行期の進んだ症例に多く発現している事を明らかにした。

Protooncogene, Ets, MMP-1,MMP-3,MMP-9,integrin, uPA, MMP-1,MMP-3,MMP-9,integrin, cyclin, cyclin, cell cycle, tumor, cell cycle, tumor differentiation, cell differentiation and differentiation. The expression of Ets-1 protein in epithelial ovarian cancer [Takai et al., Cancer,2000], epithelial ovarian cancer [Takai et al., Int J Mol Med,2002], and Elf-1 protein in epithelial ovarian cancer [Takai et al., Gynecol Oncol,2003], epithelial ovarian cancer [Takai et al., Int J Mol Med,2003], histopathology, histology, histology, histopathology, histology, histopathology, histopathology, histology, histopathology, histopathology, histology, histopathology, The cancer cells, the differentiation cells, the degree of differentiation, the degree of differentiation, and the degree of differentiation. Ets cancer, maternal cancer, ovary cancer, ovarian cancer, maternal cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, ovarian cancer, ovarian cancer, maternal cancer, maternal The cancer cell, the differentiation cell, the degree of differentiation, the degree of differentiation and the degree of differentiation. It is impossible to analyze mitogen cells (HeLa cells, HUAA cells, OMC-3 cells). The clinical symptoms were detected by Elisa, in situ hybridization, Immunohistochemistry, and the low degree of differentiation of Ets was detected by immunohistology.

项目成果

Handbook of immunohistochemistry and in situ hybridization of human carcinomas. Vol 4.

人类癌症免疫组织化学和原位杂交手册。

• 发表时间: 2006
• 作者: Akiko Ono;Takuma Fujii;et al.;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai
• 通讯作者: Noriyuki Takai

K252a inhibits proliferation of ovarian cancer cells by upregulating p21^<WAF1>

K252a 通过上调 p21^<WAF1> 抑制卵巢癌细胞增殖

• 发表时间: 2005
• 期刊: Oncol Rep 14(1)
• 作者: Akiko Ono;Takuma Fujii;et al.;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai
• 通讯作者: Noriyuki Takai

Expression of c-Ets1 protein in normal human placenta

c-Ets1蛋白在正常人胎盘中的表达

• 发表时间: 2006
• 期刊: Gynecol Obstet Invest 61
• 作者: Kaei;Nasu;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai
• 通讯作者: Noriyuki Takai

C2-ceramide exhibits antiproliferative activity and potently induces apoptosis in endometrial carcinoma

C2-神经酰胺在子宫内膜癌中表现出抗增殖活性并有效诱导细胞凋亡

• 发表时间: 2005
• 期刊: Oncol Rep 14(15)
• 作者: Akiko Ono;Takuma Fujii;et al.;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai
• 通讯作者: Noriyuki Takai

Management of patients with carcinoma of the cervix with anuric renal failure.

无尿性肾功能衰竭的宫颈癌患者的治疗。

• 发表时间: 2005
• 期刊: Int J Gynaccol Obstet 88(2)
• 作者: Akiko Ono;Takuma Fujii;et al.;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai;Noriyuki Takai
• 通讯作者: Noriyuki Takai