Induction of reparative dentin by Enamel Proteins

牙釉质蛋白诱导修复性牙本质

• 批准号: 16591921
• 负责人: NAKAMURA Yukio
• 金额: $ 2.34万
• 依托单位: Meikai University (2007)Showa University (2004-2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16591921/
• 关键词: ameloblastin; pulp; dentin; wound healing; immunohistochemistry; Enamel Matrix Proteins (EMD); type I Collagen; DSP; shenthlin

We have shown that enamel matrix derivative (EMD) enhanced reparative dentin formation was associated with a sequential expression of dentin-specific factors in the wounded pulp. Notably, in these studies, expression of pig ameloblastin was detected in pulpal cells in the wound area shortly following EMD application. To confirm a functional role for Ambn in dentinogenesis and to explore the potential use of Ambn for pulpal healing, we investigated if application of recombinant fusion Ambn (rAmbn) onto exposed pulp tissue could enhance pulpal healing and secondary dentin formation by histomorphometry and immunohistochemistry. In rAmbn-treated teeth, a substantial amount of newly formed reparative dentin was observed at the application site, completely bridging the pulpal wound. Dentin formation was also observed in Ca(OH)2 trated teeth; however, the amount of reparative dentin was significantly smaller than after rAmbn treatment. Immunohistochemistry confirmed that the new hard tissue formed was similar to dentin. The results suggest potential for rAmbn as a biologically active pulp-dressing agent for enhanced pulpal wound healing and reparative dentin formation after pulpotomy procedures.

我们已经表明，釉基质衍生物（EMD）增强修复性牙本质的形成与牙本质特异性因子在创伤牙髓中的顺序表达有关。值得注意的是，在这些研究中，在EMD应用后不久，在创伤区域的牙髓细胞中检测到猪成釉蛋白的表达。为了证实Ambn在牙本质形成中的功能作用，并探索Ambn在牙髓愈合中的潜在用途，我们通过组织形态计量学和免疫组织化学研究了重组融合Ambn（rAmbn）应用于暴露的牙髓组织是否可以增强牙髓愈合和继发性牙本质形成。在rAmbn治疗的牙齿中，在应用部位观察到大量新形成的修复性牙本质，完全桥接牙髓伤口。在Ca（OH）2处理的牙齿中也观察到牙本质形成;然而，修复性牙本质的量显著小于rAMBN处理后的量。免疫组化证实新形成的硬组织与牙本质相似。结果表明，潜在的rAmbn作为一种生物活性的牙髓敷料剂，增强牙髓伤口愈合和修复性牙本质形成后，活髓切断术程序。

项目成果

Ameloblastin Fusion Proteinsによる修復象牙質の形成誘導について

成釉细胞融合蛋白诱导修复性牙本质形成

• 发表时间: 2007
• 作者: 中村 幸生;松本 光吉
• 通讯作者: 松本 光吉

Analysis ofsurface roughness and microleakage of fissure sealants following organic debris removal with Carisolv

使用 Carisolv 去除有机碎片后窝沟封闭剂的表面粗糙度和微渗漏分析

• 发表时间: 2008
• 期刊: Journal of dentistry 36(2)
• 作者: Yamada;Y.;Hossain;M.;Shimizu;Y.;Kimura;Y.;Masuda;Y.;Nakamura;Y.;Matsumoto;K
• 通讯作者: K

Analysis of surface roughness and microleakage of fissure sealants following organic debris removal with Carisolv.

使用 Carisolv 去除有机碎片后窝沟封闭剂的表面粗糙度和微渗漏分析。

• 发表时间: 2008
• 期刊: Journal of dentistry 36(2)
• 作者: Yamada Y;Hossain M;Shimizu Y;Kimura Y;Masuda Y;Nakamura Y;Matsumoto K
• 通讯作者: Matsumoto K

Analysis of surface roughness and microleakage of fissure sealants following organic debris removal with Carisolv

使用 Carisolv 去除有机碎片后窝沟封闭剂的表面粗糙度和微渗漏分析

• 发表时间: 2008
• 期刊: Journal of dentistry 36(2)
• 作者: Yamada Y;Hossain M;Shimizu Y;Kimura Y;MasudaY;Nakamura Y;Matsumoto K
• 通讯作者: Matsumoto K

An assessment following root canal preparation by Er, Cr : YSGG laser irradiation in straight and curved roots, in vitro

Er、Cr 根管预备后的评估：体外直根和弯根的 YSGG 激光照射

• 发表时间: 2006
• 期刊: Laser Medicine and Science 21
• 作者: Jahan KM;Hossain M;Nakamura Y;Yamada Y;Kinoshita J;Matsumoto K
• 通讯作者: Matsumoto K